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Digestive Diseases and Sciences

, Volume 59, Issue 10, pp 2571–2579 | Cite as

Hepatic Steatosis Is Highly Prevalent in Hepatitis B Patients and Negatively Associated with Virological Factors

  • Man-Man Wang
  • Gong-Sui Wang
  • Feng Shen
  • Guang-Yu Chen
  • Qin Pan
  • Jian-Gao FanEmail author
Original Article

Abstract

Background and Aims

The interaction between hepatitis B virus (HBV) infection and hepatic steatosis remains unclear. We aimed to explore the trend of prevalence of hepatic steatosis and its relationship with virological factors in HBV infected patients.

Methods

Consecutive untreated patients with chronic HBV infection at Shunde Hospital between 2002 and 2011 were included. Quantification of HBV replication markers was performed by enzyme immunoassay, real-time polymerase chain reaction assay and immunohistochemical staining. Hepatic steatosis was defined as at least 5 % hepatocytes affected.

Results

A total of 3,212 patients (2,574 men) with a mean age of 32 ± 9.3 years were analyzed. Serological testing showed detectable HBsAg in all, HBeAg in 63.8 % and HBV DNA in 78.4 % of patients. Liver biopsies demonstrated HBsAg- and HBcAg-positive immunostaining in 96.6 and 71 % patients, respectively. Hepatic steatosis was present in 554 (17.3 %) patients, with annual prevalence increased over time from 8.2 to 31.8 % (trend analysis, x 2 = 51.657, P < 0.001). Compared to patients without steatosis, the percentages of serum HBeAg-positive and detectable HBV DNA, and intrahepatic HBsAg- and HBcAg-positive staining were decreased in steatosis patients (all P < 0.001). Adjusted for age and gender, intrahepatic HBsAg-positive staining remained as an independent factor associated with lower risk of steatosis (adjusted odds ratio 0.90, 95 % confidence interval 0.835, 0.971) in multivariate analysis.

Conclusions

Hepatic steatosis in HBV infected patients has been raging over the past decade, and it is negatively associated with intrahepatic expression of HBsAg. Lifestyle intervention may be needed to halt the onset of steatosis in chronic HBV infection.

Keywords

Hepatitis B virus Hepatic steatosis Nonalcoholic fatty liver disease Hepatitis B surface antigen Metabolic syndrome 

Notes

Acknowledgments

We wish to thank all patients and medical staff who participated in the study. This work was financially supported by the State Key Development Program for Basic Research of China (2012CB517500), the Program of the Shanghai Committee of Science and Technology (09140903500 &10411956300), the National Natural Science Foundation of China (81070322 &81270491), and the 100 Talents Program of the Shanghai Board of Health (XBR2011007).

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Man-Man Wang
    • 1
    • 2
  • Gong-Sui Wang
    • 3
  • Feng Shen
    • 1
  • Guang-Yu Chen
    • 1
  • Qin Pan
    • 1
  • Jian-Gao Fan
    • 1
    Email author
  1. 1.Department of Gastroenterology, Xinhua Hospital, Shanghai Key Laboratory of Children’s Digestion and NutritionShanghai Jiao Tong University School of MedicineShanghaiChina
  2. 2.Department of EndocrinologyThe Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhouChina
  3. 3.Department of Infectious DiseaseShunde First People’s Hospital Affiliated to Southern Medical UniversityShundeChina

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