Digestive Diseases and Sciences

, Volume 58, Issue 11, pp 3178–3188

MicroRNA Expression Differentiates Squamous Epithelium from Barrett’s Esophagus and Esophageal Cancer

  • Katherine S. Garman
  • Kouros Owzar
  • Elizabeth R. Hauser
  • Kristen Westfall
  • Blair R. Anderson
  • Rhonda F. Souza
  • Anna Mae Diehl
  • Dawn Provenzale
  • Nicholas J. Shaheen
Original Article

DOI: 10.1007/s10620-013-2806-7

Cite this article as:
Garman, K.S., Owzar, K., Hauser, E.R. et al. Dig Dis Sci (2013) 58: 3178. doi:10.1007/s10620-013-2806-7

Abstract

Background

Current strategies fail to identify most patients with esophageal adenocarcinoma (EAC) before the disease becomes advanced and incurable. Given the dismal prognosis associated with EAC, improvements in detection of early-stage esophageal neoplasia are needed.

Aim

We sought to assess whether differential expression of microRNAs could discriminate between squamous epithelium, Barrett’s esophagus (BE), and EAC.

Methods

We analyzed microRNA expression in a discovery cohort of human endoscopic biopsy samples from 36 patients representing normal squamous esophagus (n = 11), BE (n = 14), and high-grade dysplasia/EAC (n = 11). RNA was assessed using microarrays representing 847 human microRNAs followed by quantitative real-time polymerase chain reaction (qRT-PCR) verification of nine microRNAs. In a second cohort (n = 18), qRT-PCR validation of five miRNAs was performed. Expression of 59 microRNAs associated with BE/EAC in the literature was assessed in our training cohort. Known esophageal cell lines were used to compare miRNA expression to tissue miRNAs.

Results

After controlling for multiple comparisons, we found 34 miRNAs differentially expressed between squamous esophagus and BE/EAC by microarray analysis. However, miRNA expression did not reliably differentiate non-dysplastic BE from EAC. In the validation cohort, all five microRNAs selected for qRT-PCR validation differentiated between squamous samples and BE/EAC. Microarray results supported 14 of the previously reported microRNAs associated with BE/EAC in the literature. Cell lines did not generally reflect miRNA expression found in vivo.

Conclusions

These data indicate that miRNAs differ between squamous esophageal epithelium and BE/EAC, but do not distinguish between BE and EAC. We suggest prospective evaluation of miRNAs in patients at high risk for EAC.

Keywords

Barrett esophagus Esophageal neoplasms Microarray analysis microRNAs 

Supplementary material

10620_2013_2806_MOESM1_ESM.docx (78 kb)
Supplementary material 1 (DOCX 78 kb)

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Katherine S. Garman
    • 1
    • 2
  • Kouros Owzar
    • 6
  • Elizabeth R. Hauser
    • 2
    • 7
  • Kristen Westfall
    • 8
  • Blair R. Anderson
    • 9
  • Rhonda F. Souza
    • 3
    • 4
  • Anna Mae Diehl
    • 1
  • Dawn Provenzale
    • 1
    • 2
  • Nicholas J. Shaheen
    • 5
  1. 1.Division of Gastroenterology, Department of MedicineDuke UniversityDurhamUSA
  2. 2.Durham VA Medical CenterDurhamUSA
  3. 3.VA North Texas Health Care SystemDallasUSA
  4. 4.University of Texas Southwestern Medical CenterDallasUSA
  5. 5.University North Carolina at Chapel HillChapel HillUSA
  6. 6.Biostatistics and BioinformaticsDuke UniversityDurhamUSA
  7. 7.Center for Human GeneticsDuke UniversityDurhamUSA
  8. 8.Division of Gastroenterology, Department of MedicineDuke UniversityDurhamUSA
  9. 9.Center for Human GeneticsDuke UniversityDurhamUSA

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