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Digestive Diseases and Sciences

, Volume 58, Issue 7, pp 2028–2037 | Cite as

Low Expression of CyclinH and Cyclin-Dependent Kinase 7 Can Decrease the Proliferation of Human Esophageal Squamous Cell Carcinoma

  • Jianguo Zhang
  • Xiaojing Yang
  • Yuchan Wang
  • Hui Shi
  • Chengqi Guan
  • Li Yao
  • Xianting Huang
  • Zongmei Ding
  • Yuejiao Huang
  • Huijie Wang
  • Chun ChengEmail author
Original Article

Abstract

Background

Increased expression of cyclinH (CCNH) and cyclin-dependent kinase 7 (CDK7) has a relationship with poor prognosis in most human cancers.

Aim

Investigate the expression of CCNH and CDK7 in human esophageal squamous cell carcinoma (ESCC) and the effect of chemotherapy on their expression.

Methods

Western blotting and immunohistochemistry were used to measure the expression of CCNH and CDK7 proteins in ESCC and adjacent normal tissue in 98 patients. We use Cell Counting Kit-8 and cell flow to analyze the effects of cisplatin and interference of CCNH and CDK7 in cell cycle process.

Results

Immunohistochemical analysis showed that CCNH and CDK7 expression were significantly associated with unfavorable clinicopathologic variables. CCNH and CDK7 protein levels were elevated in ESCC tissues in comparison with adjacent normal tissues. Survival analysis revealed that CCNH and CDK7 overexpression were significantly associated with overall survival (P < 0.001). Cisplatin or interference of CCNH or CDK7 led cells to grow slowly. Overexpression of CCNH and CDK7 in TE1 cells can lead to resistance to cisplatin.

Conclusions

We can conclude that CCNH and CDK7 may play an important role in the tumorigenesis and development of ESCC. CCNH and CDK7 expression affected the chemotherapy of tumor.

Keywords

Esophageal squamous cell carcinoma CyclinH CDK7 Tumor immunology Proliferation 

Notes

Acknowledgments

This work was supported by the National Basic Research Program of China (973 Program, No. 2011CB910604, and No. 2012CB822104); the National Natural Science Foundation of China (No. 81172879, No. 81201858); Natural Scientific Foundation of Jiangsu Province Grant (No. BK2012231); A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Jianguo Zhang
    • 1
  • Xiaojing Yang
    • 1
  • Yuchan Wang
    • 1
  • Hui Shi
    • 1
  • Chengqi Guan
    • 1
  • Li Yao
    • 1
  • Xianting Huang
    • 1
  • Zongmei Ding
    • 1
  • Yuejiao Huang
    • 1
  • Huijie Wang
    • 2
  • Chun Cheng
    • 1
    Email author
  1. 1.Department of Immunology, Medical CollegeNantong UniversityNantongPeople’s Republic of China
  2. 2.Department of Medical Oncology, Fudan University Shanghai Cancer CenterFudan UniversityShanghaiPeople’s Republic of China

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