Digestive Diseases and Sciences

, Volume 58, Issue 6, pp 1717–1726 | Cite as

Changes of the Intestinal Microbiota, Short Chain Fatty Acids, and Fecal pH in Patients with Colorectal Cancer

  • Seiji Ohigashi
  • Kazuki Sudo
  • Daiki Kobayashi
  • Osamu Takahashi
  • Takuya Takahashi
  • Takashi Asahara
  • Koji Nomoto
  • Hisashi Onodera
Original Article

Abstract

Background

New molecular biology-based methods of bacterial identification are expected to help elucidate the relationship between colorectal cancer (CRC) and intestinal microbiota. Although there is increasing evidence revealing the potential role of microbiota in CRC, it remains unclear whether microbial dysbiosis is the cause or the result of CRC onset.

Aim

We investigated the changes of intestinal environments in CRC or adenoma.

Methods

We analyzed 13 groups of microbiota, 8 types of organic acids, and pH in feces obtained from the following 3 groups: individuals with CRC, adenoma, and non-adenoma. Ninety-three patients with CRC and 49 healthy individuals (22 with adenoma and 27 without adenoma) were enrolled.

Results

The counts of total bacteria (10.3 ± 0.7 vs. 10.8 ± 0.3 log10 cells/g of feces; p < 0.001), 5 groups of obligate anaerobe, and 2 groups of facultative anaerobes were significantly lower in the CRC group than in the healthy individuals. While the concentrations of short chain fatty acids (SCFAs) were significantly decreased in the CRC group, the pH was increased in the CRC group (7.4 ± 0.8 vs. 6.9 ± 0.6; p < 0.001). Comparison among the CRC, adenoma, and non-adenoma groups revealed that fecal SCFAs and pH in the adenoma group were intermediate to the CRC group and the non-adenoma group. Within the CRC group, no differences in microbiota or organic acids were observed among Dukes stages.

Conclusions

CRC patients showed significant differences in the intestinal environment, including alterations of microbiota, decreased SCFAs, and elevated pH. These changes are not a result of CRC progression but are involved in CRC onset.

Keywords

Colorectal cancer Adenoma Microbiota Short chain fatty acid Fecal pH 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Seiji Ohigashi
    • 1
  • Kazuki Sudo
    • 1
  • Daiki Kobayashi
    • 2
  • Osamu Takahashi
    • 3
  • Takuya Takahashi
    • 4
  • Takashi Asahara
    • 4
  • Koji Nomoto
    • 4
  • Hisashi Onodera
    • 1
  1. 1.Department of Gastroenterological SurgerySt. Luke’s International HospitalTokyoJapan
  2. 2.Department of General Internal MedicineSt. Luke’s International HospitalTokyoJapan
  3. 3.Center for Clinical EpidemiologySt. Luke’s Life Science InstituteTokyoJapan
  4. 4.Yakult Central Institute for Microbiological ResearchKunitachi-shiJapan

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