Digestive Diseases and Sciences

, Volume 57, Issue 6, pp 1496–1503 | Cite as

Gastroprotective Effect of NaHS Against Mucosal Lesions Induced by Ischemia–Reperfusion Injury in Rat

  • Seyyed Ali Mard
  • Niloofar Neisi
  • Ghasem Solgi
  • Maryam Hassanpour
  • Marjan Darbor
  • Maryam Maleki
Original Article



Hydrogen sulfide (H2S) has been shown to display anti-inflammatory and antioxidant activities.


This study was designed to investigate the gastroprotective effect of sodium hydrosulfide (NaHS) on gastric mucosal lesions induced by ischemia–reperfusion (I/R) injury in rats and to determine the possible mechanism involved.


Fifty-sex male Wistar rats were randomly assigned into sham, control (I/R injury), propargylglycine (PAG)-, l-cysteine-, and NaHS-treated groups. To induce I/R lesions, the celiac artery was first clamped for 30 min (ischemia phase), followed by removal of the clamp artery to allow reperfusion for 3 h. Treated rats received PAG [50 mg/kg, intravenous (i.v.)] or NaHS (160, 320, or 640 ng/kg, i.v.) 5 min before reperfusion. The effect of l-cysteine pretreatment was also investigated. Plasma levels of cytokines and cortisol were measured by an enzyme-linked immunosorbent assay. The gastric tissue samples were collected to quantify the mRNA expression of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and transforming growth factor (TGF-β) by quantitative real-time PCR.


The total area of gastric lesions significantly decreased following the administration of NaHS and l-cysteine. The highest area of mucosal lesions was observed in PAG-treated rats. The mRNA expression and plasma levels of IL-1β and TNF-α were significantly decreased in l-cysteine- and NaHS-treated rats in a dose-dependent manner. Slightly increased levels of TGF-β were observed in these test groups, but the difference was not statistically significant compared with the other groups. The plasma level of cortisol was also not affected by NaHS treatment.


Our findings indicate that a possible mechanism for the gastroprotective effect of H2S could be through the decreased mRNA expression and plasma release of proinflammatory cytokines.


NaHS I/R injury l-cysteine Proinflammatory cytokines Quantitative real-time PCR Rat 



The authors thank the vice-chancellor of research and technology of Ahvaz Jundi Shapour University of Medical Sciences for financial support (grant no. PRC60) and Professor William Winlow (Universities of Central Lancashire and Liverpool) for his comments on the manuscript.

Conflict of interest



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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Seyyed Ali Mard
    • 1
  • Niloofar Neisi
    • 2
  • Ghasem Solgi
    • 3
  • Maryam Hassanpour
    • 1
  • Marjan Darbor
    • 1
  • Maryam Maleki
    • 1
  1. 1.Department of Physiology, Physiology Research Center (PRC), Research institute for infectious diseases of digestive system, School of MedicineAhvaz Jundi Shapur University of Medical SciencesAhvazIran
  2. 2.Research institute for infectious diseases of digestive system and Department of Virology, The school of medicineAhvaz Jundi Shapur University of Medical SciencesAhvazIran
  3. 3.Department of Immunology, School of MedicineHamadan University of Medical Sciences (UMSHA)HamadanIran

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