Digestive Diseases and Sciences

, 56:2818

Adiponectin and Plant-Derived Mammalian Adiponectin Homolog Exert a Protective Effect in Murine Colitis

  • Violeta Arsenescu
  • Meena L. Narasimhan
  • Tuna Halide
  • Ray A. Bressan
  • Chiara Barisione
  • Donald A. Cohen
  • Willem J. S. de Villiers
  • Razvan Arsenescu
Original Article



Hypoadiponectinemia has been associated with states of chronic inflammation in humans. Mesenteric fat hypertrophy and low adiponectin have been described in patients with Crohn’s disease. We investigated whether adiponectin and the plant-derived homolog, osmotin, are beneficial in a murine model of colitis.


C57BL/6 mice were injected (i.v.) with an adenoviral construct encoding the full-length murine adiponectin gene (AN+DSS) or a reporter—LacZ (Ctr and V+DSS groups) prior to DSS colitis protocol. In another experiment, mice with DSS colitis received either osmotin (Osm+DSS) or saline (DSS) via osmotic pumps. Disease progression and severity were evaluated using body weight, stool consistency, rectal bleeding, colon lengths, and histology. In vitro experiments were carried out in bone marrow-derived dendritic cells.


Mice overexpressing adiponectin had lower expression of proinflammatory cytokines (TNF, IL-1β), adipokines (angiotensin, osteopontin), and cellular stress and apoptosis markers. These mice had higher levels of IL-10, alternative macrophage marker, arginase 1, and leukoprotease inhibitor. The plant adiponectin homolog osmotin similarly improved colitis outcome and induced robust IL-10 secretion. LPS induced a state of adiponectin resistance in dendritic cells that was reversed by treatment with PPARγ agonist and retinoic acid.


Adiponectin exerted protective effects during murine DSS colitis. It had a broad activity that encompassed cytokines, chemotactic factors as well as processes that assure cell viability during stressful conditions. Reducing adiponectin resistance or using plant-derived adiponectin homologs may become therapeutic options in inflammatory bowel disease.


Adiponectin Inflammatory bowel disease Mammalian adiponectin homolog PPARγ agonists Dendritic cells Inflammatory bowel disease 





Angiotensin converting enzyme




Angiotensin receptor 1a


Endoplasmic reticulum-binding protein (Hsp70)


Caspase 12


Chemokine (C–C motif), receptor2


Cluster of differentiation 14


C/EBP homologous protein


Cyclooxygenase 2


Dendritic cells


Dextran sodium sulfate


Endoplasmic reticulum


Inflammatory bowel disease


Interleukin 10




Monocyte chemotactic protein-1


Nucleotide-binding oligomerization domain containing 2


Proliferating cell nuclear antigen


prostaglandin E2


Peroxisome proliferator-activated receptor γ


p38 mitogen-activated protein kinase


Secretory leukoprotease inhibitor

Th1, 2

T helper cell type 1, 2


Toll-like receptor


Tumor necrosis factor α


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Violeta Arsenescu
    • 1
    • 2
  • Meena L. Narasimhan
    • 3
  • Tuna Halide
    • 4
  • Ray A. Bressan
    • 3
    • 6
    • 7
  • Chiara Barisione
    • 5
  • Donald A. Cohen
    • 4
  • Willem J. S. de Villiers
    • 1
    • 2
  • Razvan Arsenescu
    • 1
    • 4
  1. 1.Division of Digestive Diseases and Nutrition, Microbiology, Immunology and Molecular GeneticsUniversity of Kentucky Medical CenterLexingtonUSA
  2. 2.Graduate Center for Nutritional SciencesUniversity of KentuckyLexingtonUSA
  3. 3.Plant Stress Genomics Research CenterKing Abdullah University of Science and TechnologyThuwalKingdom of Saudi Arabia
  4. 4.Microbiology, Immunology and Molecular GeneticsUniversity of KentuckyLexingtonUSA
  5. 5.Research Center of Cardiovascular BiologyUniversity of GenoaLiguriaItaly
  6. 6.Division of Applied Sciences, WCU programGyeongsang National UniversityJinjuSouth Korea
  7. 7.Departments of Horticulture and Landscape ArchitecturePurdue UniversityWest LafayetteUSA

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