Digestive Diseases and Sciences

, Volume 55, Issue 10, pp 2805–2813

Granulocyte Colony-Stimulating Factor Enhances Bone Marrow Mononuclear Cell Homing to the Liver in a Mouse Model of Acute Hepatic Injury

  • Shi-Zhu Jin
  • Xiang-Wei Meng
  • Xun Sun
  • Ming-Zi Han
  • Bing-Rong Liu
  • Xin-Hong Wang
  • Li-Ying Sun
  • Qi Huang
  • Rui-Bo Zhao
  • Xiang Ban
  • Hong-Ying Yu
  • Hong-Wei Yu
Original Article

Abstract

Background

Experiments have reported that granulocyte colony stimulating factor (G-CSF) can mobilize stem cells. However, few studies have examined the effect of G-CSF on bone marrow mononuclear cell (BMMC) mobilization, in particular regarding their capability to home to acutely injured liver.

Aims

The aim of this study was to evaluate the effort of G-CSF on BMMC homing to the liver following chemically-induced hepatic failure.

Methods

BMMC were isolated from mice, pre-labeled with PKH26 and infused into the mice in which hepatic injury had been induced followed by administration of G-CSF or vehicle. Livers were studied by fluorescent microscopy after transplantation of pre-labeled BMMC.

Results

PKH26 labeled cells were found in liver tissue at 102 ± 10 cells/high power field in the BMMC+G-CSF group and 30 ± 5 cells/high power field in the BMMC group, but none in the G-CSF group and the control group (P < 0.05). In the former two groups the majority of PKH26 labeled cells colocalized with proliferative cell nuclear antigen (PCNA). The number of PCNA positive cells in the BMMC+G-CSF group was 20 ± 4 cells/high power field, while in the BMMC group it was 14 ± 2 cells/high power field, in the G-CSF group 12 ± 2 cells/high power field, and 8 ± 1 cells/high power field in the control group. Moreover, albumin expression was increased in the BMMC+G-CSF treated group (149 ± 7/high power field) relative to the BMMC group (48 ± 6/high power field), the G-CSF group (44 ± 5/high power field) and the vehicle group (30 ± 6/high power field), with the former three groups showing elevated levels as compared to vehicle control (30 ± 6) (P < 0.05).

Conclusion

Transplanted BMMC may home to injured liver, which appears to be enhanced by G-CSF administration.

Keywords

Granulocyte colony stimulating factor Bone marrow mononuclear cell Acute liver failure Transplantation Mobilization Autologous 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Shi-Zhu Jin
    • 1
  • Xiang-Wei Meng
    • 1
  • Xun Sun
    • 2
  • Ming-Zi Han
    • 3
  • Bing-Rong Liu
    • 3
  • Xin-Hong Wang
    • 1
  • Li-Ying Sun
    • 3
  • Qi Huang
    • 4
  • Rui-Bo Zhao
    • 4
  • Xiang Ban
    • 4
  • Hong-Ying Yu
    • 3
  • Hong-Wei Yu
    • 5
  1. 1.Department of Gastroenterology, First HospitalJilin UniversityChangchunChina
  2. 2.Department of Pathology, First HospitalJilin UniversityChangchunChina
  3. 3.Department of Gastroenterology and Hepatology, Second Affiliated HospitalHarbin Medical UniversityHarbinChina
  4. 4.Teaching Experiment Center of MorphologyHarbin Medical UniversityHarbinChina
  5. 5.Department of Histology and EmbryologyHarbin Medical UniversityHarbinChina

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