Digestive Diseases and Sciences

, Volume 55, Issue 9, pp 2505–2513

Lactobacillus plantarum Prevents the Upregulation of Adhesion Molecule Expression in an Experimental Colitis Model

  • Zhao-Xin Chu
  • Hong-Qi Chen
  • Yan-Lei Ma
  • Yu-Kun Zhou
  • Ming Zhang
  • Peng Zhang
  • Huan-Long Qin
Original Article

DOI: 10.1007/s10620-009-1063-2

Cite this article as:
Chu, ZX., Chen, HQ., Ma, YL. et al. Dig Dis Sci (2010) 55: 2505. doi:10.1007/s10620-009-1063-2

Abstract

Background

Lactobacillus consumption has been shown to attenuate the severity of experimental colitis. Whether the effects of Lactobacillus on colitis are related to modulation of leukocyte recruitment into the inflamed intestine is unclear.

Aims

To investigate the effect of Lactobacillus plantarum daily intragastric administration on lymphocyte homing and intestinal inflammation in interleukin 10 (IL-10) knockout mice, an experimental model of colitis.

Methods

Two groups of ten IL-10 knockout mice were fed phosphate buffered saline containing Lactobacillus plantarum 1258 or unmodified vehicle for 4 weeks. Two groups of ten wild-type mice were used as controls. At killing, the bowels were histologically scored and evaluated by transmission electron microscopy. Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) and intercellular adhesion molecule 1 (ICAM-1) expression were determined by immunohistochemistry. The Levels of proinflammatory cytokines, tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ) were determined by ELISA. In addition, levels of CD3, α4β7, ICAM-1, and MAdCAM-1 were determined by reverse-transcription polymerase chain reaction and Western blot.

Results

L. plantarum treatment improved the histological damage score in KO mice compared to untreated KO mice. L. plantarum significantly attenuated the expression of MAdCAM-1, ICAM-1, CD3, and α4β7, but did not affect the levels of TNF-α and IFN-γ when treated KO mice were compared to untreated KO mice.

Conclusions

L. plantarum interfered with the upregulation of adhesion molecules observed in IL-10 knockout mice compared to wild-type mice, attenuating the symptoms of colitis.

Keywords

Probiotics Cell adhesion molecules Inflammatory bowel disease (IBD) Interleukin-10 knockout mice Lymphocyte homing 

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Zhao-Xin Chu
    • 1
  • Hong-Qi Chen
    • 1
  • Yan-Lei Ma
    • 1
  • Yu-Kun Zhou
    • 1
  • Ming Zhang
    • 1
  • Peng Zhang
    • 1
  • Huan-Long Qin
    • 1
  1. 1.Department of SurgeryThe Sixth People’s Hospital Affiliated to Shanghai Jiao Tong UniversityShanghaiChina

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