Vitamin D Receptor Gene ApaI Polymorphism Is Associated with Susceptibility to Colorectal Cancer
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The purpose of this study was to investigate vitamin D receptor (VDR) gene ApaI and TaqI polymorphisms in relation to the risk of colorectal cancer (CRC) in the Iranian population.
Two single nucleotide polymorphisms in intron 8 (ApaI) and exon 9 (TaqI) of the VDR gene were genotyped in 160 subjects with CRC and 180 controls by use of the PCR–RFLP method.
The VDR ApaI genotype distribution differed significantly between subjects with CRC and controls. The ApaI “aa” genotype compared with “AA” genotype was associated with a 2.3-fold increased risk of CRC (P = 0.014; OR = 2.25, 95% CI = 1.18–4.28) and the difference remained significant after adjustment for age, BMI, sex, and smoking status (P = 0.014; OR = 2.32, 95% CI = 1.19–4.54). Furthermore, the ApaI “a” allele was significantly overrepresented in subjects with CRC than controls (P = 0.014; OR = 1.48, 95% CI = 1.08–2.02).
These findings suggest that VDR ApaI genotype “aa” is associated with increased risk of CRC in the Iranian population. However, these data remain to be confirmed in larger studies and in other populations.
KeywordsColorectal cancer Vitamin D receptor gene Single nucleotide polymorphism PCR–RFLP
The authors are grateful to Dr Somaye Ghiasi and to Mrs Shohreh Almasi for her technical assistance. The authors also thank all patients and healthy blood donors for providing blood samples. This work was supported by a grant from the Research Center of Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences.
- 10.Theodoratou E, Farrington SM, Tenesa A, et al. Modification of the inverse association between dietary vitamin D intake and colorectal cancer risk by a FokI variant supports a chemoprotective action of vitamin D intake mediated through VDR binding. Int J Cancer. 2008;123:2170–2179.CrossRefPubMedGoogle Scholar
- 13.Talor JA, Hirvonen A, Watson M, Pittman G, Mohler JL, Bell DA. Association of prostate cancer with vitamin D receptor gene polymorphism. Cancer Res. 1996;56:4108–4110.Google Scholar