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Digestive Diseases and Sciences

, Volume 55, Issue 7, pp 2008–2013 | Cite as

Vitamin D Receptor Gene ApaI Polymorphism Is Associated with Susceptibility to Colorectal Cancer

  • Touraj MahmoudiEmail author
  • Seyed Reza Mohebbi
  • Mohamad Amin Pourhoseingholi
  • Seyed Reza Fatemi
  • Mohammad Reza Zali
Original Article

Abstract

Objectives

The purpose of this study was to investigate vitamin D receptor (VDR) gene ApaI and TaqI polymorphisms in relation to the risk of colorectal cancer (CRC) in the Iranian population.

Methods

Two single nucleotide polymorphisms in intron 8 (ApaI) and exon 9 (TaqI) of the VDR gene were genotyped in 160 subjects with CRC and 180 controls by use of the PCR–RFLP method.

Results

The VDR ApaI genotype distribution differed significantly between subjects with CRC and controls. The ApaI “aa” genotype compared with “AA” genotype was associated with a 2.3-fold increased risk of CRC (P = 0.014; OR = 2.25, 95% CI = 1.18–4.28) and the difference remained significant after adjustment for age, BMI, sex, and smoking status (P = 0.014; OR = 2.32, 95% CI = 1.19–4.54). Furthermore, the ApaI “a” allele was significantly overrepresented in subjects with CRC than controls (P = 0.014; OR = 1.48, 95% CI = 1.08–2.02).

Conclusions

These findings suggest that VDR ApaI genotype “aa” is associated with increased risk of CRC in the Iranian population. However, these data remain to be confirmed in larger studies and in other populations.

Keywords

Colorectal cancer Vitamin D receptor gene Single nucleotide polymorphism PCR–RFLP 

Notes

Acknowledgments

The authors are grateful to Dr Somaye Ghiasi and to Mrs Shohreh Almasi for her technical assistance. The authors also thank all patients and healthy blood donors for providing blood samples. This work was supported by a grant from the Research Center of Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Touraj Mahmoudi
    • 1
    • 2
    Email author
  • Seyed Reza Mohebbi
    • 1
  • Mohamad Amin Pourhoseingholi
    • 1
  • Seyed Reza Fatemi
    • 1
  • Mohammad Reza Zali
    • 1
  1. 1.Research Center for Gastroenterology and Liver DiseasesShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Research Center for Gastroenterology and Liver Diseases (RCGLD)Shahid Beheshti University of Medical SciencesTehranIran

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