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Digestive Diseases and Sciences

, Volume 55, Issue 7, pp 1866–1877 | Cite as

Intestinal Ischemic Preconditioning After Ischemia/Reperfusion Injury in Rat Intestine: Profiling Global Gene Expression Patterns

  • Stacey D. Moore-Olufemi
  • Shodimu-Emmanuel Olufemi
  • Steve Lott
  • Norio Sato
  • Rosemary A. Kozar
  • Frederick A. Moore
  • Ravi S. Radhakrishnan
  • Shinil Shah
  • Fernando Jimenez
  • Bruce C. Kone
  • Charles S. CoxJr.
Original Article

Abstract

Objective

Intestinal ischemia/reperfusion (IR) injury involves activation of inflammatory mediators, mucosal necrosis, ileus, and alteration in a variety of gene products. Ischemic preconditioning (IPC) reduced all the effects of intestinal injury seen in IR. In an effort to investigate the molecular mechanisms responsible for the protective effects afforded by IPC, we sought to characterize the global gene expression pattern in rats subjected to IPC in the setting of IR injury.

Methods

Rats were randomized into five groups: (1) Sham, (2) IPC only (3) IR, (4) Early IPC + IR (IPC → IR), and (5) Late IPC + IR (IPC → 24 h → IR). At 6 h after reperfusion, ileum was harvested for total RNA isolation, pooled, and analyzed on complementary DNA (cDNA) microarrays with validation using real-time polymerase chain reaction (PCR). Significance Analysis of Microarray (SAM) software was used to determine statistically significant changes in gene expression.

Results

Early IPC + IR had 5,167 induced and 4 repressed genes compared with the other groups. SAM analysis revealed 474 out of 10,000 genes differentially expressed among the groups. Early and Late IPC + IR had more genes involved in redox hemostasis, the immune/inflammatory response, and apoptosis than either the IPC only or IR alone groups.

Conclusion

The transcriptional profile suggests that IPC exerts its protective effects by regulating the gene response to injury in the intestine.

Keywords

Ischemia/reperfusion Gene expression Intestine Preconditioning 

Notes

Acknowledgments

This work was supported by NIGMS grants T32 GM 08792, P50 GM38529, the Department of Defense “DREAMS” grant (B.C.K.), and endowment funds from The James T. and Nancy B. Willerson Chair (B.C.K.).

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Stacey D. Moore-Olufemi
    • 1
    • 5
  • Shodimu-Emmanuel Olufemi
    • 7
  • Steve Lott
    • 8
  • Norio Sato
    • 2
    • 5
  • Rosemary A. Kozar
    • 2
    • 5
  • Frederick A. Moore
    • 2
    • 5
  • Ravi S. Radhakrishnan
    • 2
    • 5
  • Shinil Shah
    • 2
  • Fernando Jimenez
    • 1
  • Bruce C. Kone
    • 3
    • 4
    • 5
    • 6
  • Charles S. CoxJr.
    • 1
    • 5
  1. 1.Department of Pediatric SurgeryUniversity of Texas Medical School at HoustonHoustonUSA
  2. 2.Department of SurgeryUniversity of Texas Medical School at HoustonHoustonUSA
  3. 3.Department of Internal MedicineUniversity of Texas Medical School at HoustonHoustonUSA
  4. 4.Department of Integrative Biology and PharmacologyUniversity of Texas Medical School at HoustonHoustonUSA
  5. 5.Department of Trauma Research CenterUniversity of Texas Medical School at HoustonHoustonUSA
  6. 6.The Brown Foundation Institute of Molecular Medicine for the Prevention of Human DiseasesHoustonUSA
  7. 7.Department of Cancer GeneticsM.D. Anderson Cancer CenterHoustonUSA
  8. 8.Department of Molecular GeneticsM.D. Anderson Cancer CenterHoustonUSA

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