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Gastroprotective Action of Cochinchina Momordica Seed Extract Is Mediated by Activation of CGRP and Inhibition of cPLA2/5-LOX Pathway

  • Jung Mook Kang
  • Nayoung KimEmail author
  • Bongcheol Kim
  • Joo-Hyon Kim
  • Bong-Yong Lee
  • Ji Hyun Park
  • Mi Kyoung Lee
  • Hye Seung Lee
  • In-Jin Jang
  • Joo Sung Kim
  • Hyun Chae Jung
  • In Sung Song
Original Article

Abstract

Cochinchina momordica seed extract (SK-MS10), which is composed of the major compounds momordica saponins, has been evaluated for its gastroprotective effects in rat models of acute gastric mucosal damage. Ethanol and water immersion restraint stress (WRS) induced gastric damage, including hemorrhages and edema, was significantly attenuated by pretreatment with SK-MS10. In addition, SK-MS10 reduced increases of mucosal myeloperoxidase (MPO), IL-1β, and TNFα levels and the expression of cPLA2, and 5-LOX induced by ethanol or WRS. SK-MS10 also increased hexosamine, adherent mucus, and the expression of MUC5AC. Furthermore, SK-MS10 enhanced the mucosal expression of the CGRP gene and its serum levels. N G-methyl L-arginine (L-NMMA) or capsaicin desensitization reversed the SK-MS10-induced gastroprotection effect. These results suggest that SK-MS10 is a gastroprotective agent against acute gastric mucosal damage by suppressing proinflammatory cytokines, down-regulating cPLA2, 5-LOX, and increasing the synthesis of mucus. Furthermore, CGRP-NO pathway was found to play an important role in these gastroprotective effects of SK-MS10.

Keywords

Cochinchina momordica Calcitonin gene-related peptide Nitric oxide Gastroprotection 

Notes

Acknowledgments

This study was supported by the grant no. 06-2007-134-0 from the Seoul National University Hospital Research Fund.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jung Mook Kang
    • 1
  • Nayoung Kim
    • 1
    • 2
    Email author
  • Bongcheol Kim
    • 3
  • Joo-Hyon Kim
    • 3
  • Bong-Yong Lee
    • 3
  • Ji Hyun Park
    • 1
  • Mi Kyoung Lee
    • 1
  • Hye Seung Lee
    • 4
  • In-Jin Jang
    • 5
  • Joo Sung Kim
    • 1
  • Hyun Chae Jung
    • 1
  • In Sung Song
    • 1
  1. 1.Department of Internal Medicine and Liver Research InstituteSeoul National University College of MedicineSeoulSouth Korea
  2. 2.Department of Internal MedicineSeoul National University Bundang HospitalSeongnam, Gyeonggi-doSouth Korea
  3. 3.Life Science R&D Center, SK ChemicalsSuwon, Gyeonggi-doSouth Korea
  4. 4.Department of PathologySeoul National University Bundang HospitalSeongnam, Gyeonggi-doSouth Korea
  5. 5.Department of PharmacologySeoul National University College of MedicineSeoulSouth Korea

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