Digestive Diseases and Sciences

, Volume 54, Issue 2, pp 246–254 | Cite as

Antibacterial Action of Selenium-Enriched Probiotics Against Pathogenic Escherichia coli

  • Jiajun Yang
  • Kehe Huang
  • Shunyi Qin
  • Xianshi Wu
  • Zhiping Zhao
  • Fu Chen
Original Article


The purpose of this study was to evaluate the inhibitory activity of selenium-enriched probiotics against pathogenic Escherichia coli (E. coli) in vitro and in vivo. Escherichia coli was co-cultured in vitro with each probiotic strain individually, and a mixture of the four strains and its population was counted at various time points. We also collected a cell-free culture supernatant (CFCS) of each probiotic strain and the four-strain mix to examine their antibacterial activity, using the cylinder plate method. Results demonstrated that co-culture with probiotics significantly reduced the number of E. coli. The different sizes of the inhibition zones made by each CFCS proved that E. coli was inhibited by the metabolites of the probiotics. In vivo, Kunming mice were allocated to different groups supplemented with selenium-enriched and other probiotics. After 28 days, the mice were inoculated with pathogenic E. coli so that we could compare mortality rates and inspect other indexes of each treatment. The mortality of the group with selenium-enriched probiotics was the lowest. In addition, the organic antioxidant status improved, immunity was fortified, and the internal environment of the intestinal tract was enhanced with selenium-enriched probiotic supplementation. In conclusion, selenium-enriched probiotics can strongly antagonize pathogenic E. coli in vitro and in vivo.


Selenium Probiotics Antibacterial activity In vitro and in vivo 



The work was supported by the National Natural Science Foundation of China (grant no. 30671547), the Ph.D. Programs Foundation of the Ministry of Education of China (grant no. 20070307005), and the Natural Science Foundation of Jiangsu Province (grant no. BK2006144).


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Jiajun Yang
    • 1
  • Kehe Huang
    • 1
    • 2
  • Shunyi Qin
    • 1
  • Xianshi Wu
    • 1
  • Zhiping Zhao
    • 1
  • Fu Chen
    • 1
  1. 1.Institute of Nutritional and Metabolic DisordersNanjing Agricultural UniversityNanjingChina
  2. 2.College of Veterinary MedicineNanjing Agricultural UniversityNanjingChina

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