Digestive Diseases and Sciences

, Volume 53, Issue 5, pp 1271–1277 | Cite as

Daily Intake of High Dietary Fiber Slows Accelerated Colonic Transit Induced by Restrain Stress in Rats

  • Toku TakahashiEmail author
  • Yukiomi Nakade
  • Hiroyuki Fukuda
  • Kiyoshi Tsukamoto
  • Christopher Mantyh
  • Theodore. N. Pappas
Original Paper


Background Stress and central corticotropin releasing factor (CRF) are contributing factors to the pathogenesis of irritable bowel syndrome (IBS). It has been shown that restraint stress and central CRF stimulate colonic motility in rats. Short-chain fatty acids (SCFAs) are produced by bacterial fermentation from dietary fibers. It is controversial whether daily intake of dietary fiber is beneficial for patients with diarrhea-predominant IBS. We studied whether dietary fiber intake affects colonic transit and motility stimulated by restraint stress and central CRF in rats. Methods Corn starch is digested almost completely in the small intestine, while potato starch shows substantial resistance to α-amylase and its consumption leads to stimulation of large-bowel fermentation. Four weeks after a corn starch, potato starch, or usual diet (rat chow), colonic transit and motility stimulated by restraint stress and intracisternal (ic) injection of CRF were studied. Fecal concentration of SCFAs in the cecum was measured by high-performance liquid chromatography (HPLC). Results Four-week treatment with potato starch diet significantly increased cecal concentrations of SCFAs, compared to treatment with corn starch diet. Accelerated colonic transit induced by stress and ic injection of CRF (1 μg) were significantly attenuated in rats receiving potato starch diet compared to rats receiving corn starch diet. The incidence of unformed stool (diarrhea) induced by stress and CRF was also reduced in rats receiving potato starch diet compared to rats receiving corn starch diet and usual diet. Conclusion It is suggested that daily intake of a high-fiber diet may prevent stress- and CRF-induced acceleration of colonic transit and diarrhea. This study may contribute to treatment for the patients of diarrhea-predominant IBS.


Constipation Corticotropin releasing factor Diarrhea Irritable bowel syndrome Short-chain fatty acids 



Corticotropin releasing factor








Irritable bowel syndrome



This study was supported in part by the National Institute of Diabetes and Digestive and Kidney Diseases (RO1 DK55808 and RO1 DK62768, T.T.).


  1. 1.
    Camilleri M, Ford MJ (1998) Review article: colonic sensorimotor physiology in health, and its alteration in constipation and diarrhoeal disorders. Aliment Pharmacol Ther 12:287–302PubMedCrossRefGoogle Scholar
  2. 2.
    Proano M, Camilleri M, Phillips SF, Brown ML, Thomforde GM (1990) Transit of solids through the human colon: regional quantification in the unprepared bowel. Am J Physiol 258:G856–G862PubMedGoogle Scholar
  3. 3.
    Takahashi T, Owyang C (1998) Regional differences in the nitrergic innervation between the proximal and the distal colon in rats. Gastroenterology 115:1504–1512PubMedCrossRefGoogle Scholar
  4. 4.
    Vassallo M, Camilleri M, Phillips SF, Brown ML, Chapman NJ, Thomforde GM (1992) Transit through the proximal colon influences stool weight in the irritable bowel syndrome. Gastroenterology 102:102–108PubMedGoogle Scholar
  5. 5.
    Fukudo S, Nomura T, Hongo M (1998) Impact of corticotropin-releasing hormone on gastrointestinal motility and adrenocorticotropic hormone in normal controls and patients with irritable bowel syndrome. Gut 42:845–849PubMedCrossRefGoogle Scholar
  6. 6.
    Lenz HJ, Raedler A, Greten H, Vale WW, Rivier JE (1988) Stress-induced gastrointestinal secretory and motor responses in rats are mediated by endogenous corticotropin-releasing factor. Gastroenterology 95:1510–1517PubMedGoogle Scholar
  7. 7.
    Miyata K, Ito H, Fukudo S (1998) Involvement of the 5-HT3 receptor in CRH-induce defecation in rats. Am J Physiol 274:G827–G831PubMedGoogle Scholar
  8. 8.
    Lenz HJ, Burlage M, Raedler A, Greten H (1988) Central nervous system effects of corticotropin-releasing factor on gastrointestinal transit in the rat. Gastroenterology 94:598–602PubMedGoogle Scholar
  9. 9.
    Lewis MW, Hermann GE, Rogers RC, Travagli RA (2002) In vitro and in vivo analysis of the effects of corticotropin releasing factor on rat dorsal vagal complex. J Physiol 543:135–146PubMedCrossRefGoogle Scholar
  10. 10.
    Tache Y, Martinez V, Million M, Wang L (2001) Stress and the gastrointestinal tract III. Stress-related alterations of gut motor function: role of brain corticotropin-releasing factor receptors. Am J Physiol Gastrointest Liver Physiol 280:G173–G177PubMedGoogle Scholar
  11. 11.
    Miyata K, Kamato T, Nishida A, Ito H, Yuki H, Yamano M, Tsutsumi R, Katsuyama Y, Honda K (1992) Role of the serotonin3 receptor in stress-induced defecation. J Pharmacol Exp Ther 261:297–303PubMedGoogle Scholar
  12. 12.
    Nakade Y, Fukuda H, Iwa M, Pappas T, Takahashi T (2005) Restraint stress-induced acceleration of colonic transit is mediated via a central CRF and intraluminal 5-HT3 receptors in conscious rats. Gastroenterology 128:A115Google Scholar
  13. 13.
    Fukumoto S, Tatewaki M, Yamada T, Fujimiya M, Mantyh C, Voss M, Eubanks S, Harris M, Pappas TN, Takahashi T (2003) Short-chain fatty acids stimulate colonic transit via intraluminal 5-HT release in rats. Am J Physiol Regul Integr Comp Physiol 284:R1269–R1276PubMedGoogle Scholar
  14. 14.
    Cann PA, Read NW, Holdsworth CD (1984) What is the benefit of coarse wheat bran in patients with irritable bowel syndrome? Gut 25:168–173PubMedCrossRefGoogle Scholar
  15. 15.
    Manning AP, Heaton KW, Harvey RF (1977) Wheat fibre and irritable bowel syndrome. A controlled trial. Lancet 2:417–418PubMedCrossRefGoogle Scholar
  16. 16.
    Mathers JC, Smith H, Carter S (1997) Dose-response effects of raw potato starch on small-intestinal escape, large-bowel fermentation and gut transit time in the rat. Br J Nutr 78:1015–1029PubMedCrossRefGoogle Scholar
  17. 17.
    Williams CL, Peterson JM, Villar RG, Burks TF (1987) Corticotropin-releasing factor directly mediates colonic responses to stress. Am J Physiol 253:G582–G586PubMedGoogle Scholar
  18. 18.
    Takahashi T, Mizuta Y, Owyang C (2000) Orphanin FQ, but not dynorphin A, accelerates colonic transit in rats. Gastroenterology 119:71–79PubMedCrossRefGoogle Scholar
  19. 19.
    Araki Y, Fujiyama Y, Andoh A, Koyama S, Kanauchi O, Bamba T (2000) The dietary combination of germinated barley foodstuff plus Clostridium butyricum suppresses the dextran sulfate sodium-induced experimental colitis in rats. Scand J Gastroenterol 35:1060–1067PubMedCrossRefGoogle Scholar
  20. 20.
    Cummings JH, Rombeau JL, Sakata T (1995) Physiological and clinical aspects of short-chain fatty acids. Cambridge University Press, CambridgeGoogle Scholar
  21. 21.
    Mortensen PB, Clausen MR (1996) Short-chain fatty acids in the human colon: relation to gastrointestinal health and disease. Scand J Gastroenterol Suppl 216:132–148PubMedCrossRefGoogle Scholar
  22. 22.
    Treem WR, Ahsan N, Kastoff G, Hyams JS (1996) Fecal short-chain fatty acids in patients with diarrhea-predominant irritable bowel syndrome: in vitro studies of carbohydrate fermentation. J Pediatr Gastroenterol Nutr 23:280–286PubMedCrossRefGoogle Scholar
  23. 23.
    Mortensen PB, Andersen JR, Arffmann S, Krag E (1987) Short-chain fatty acids and the irritable bowel syndrome: the effect of wheat bran. Scand J Gastroenterol 22:185–192PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Toku Takahashi
    • 1
    • 2
    Email author
  • Yukiomi Nakade
    • 1
  • Hiroyuki Fukuda
    • 1
  • Kiyoshi Tsukamoto
    • 1
  • Christopher Mantyh
    • 1
  • Theodore. N. Pappas
    • 1
  1. 1.Department of SurgeryDuke University Medical Center and Durham Veterans Affairs Medical CenterDurhamUSA
  2. 2.Zablocki VA Medical CenterMilwaukeeUSA

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