Digestive Diseases and Sciences

, Volume 53, Issue 10, pp 2687–2696 | Cite as

Alanyl-Glutamine and Glutamine Supplementation Improves 5-Fluorouracil-Induced Intestinal Epithelium Damage In Vitro

  • Manuel B. Braga-Neto
  • Cirle A. Warren
  • Reinaldo B. Oriá
  • Manuel S. Monteiro
  • Andressa A. S. Maciel
  • Gerly A. C. Brito
  • Aldo A. M. Lima
  • Richard L. Guerrant
Original Paper

Abstract

In this study, we have examined the role of glutamine derivatives in reducing 5-fluorouracil (5-FU)-induced epithelial damage in an undifferentiated crypt intestinal cell line, IEC-6. In this model, we have investigated proliferation indirectly by detecting the enzyme-derived formazan dye from the tetrazolium salt WST-1 in viable cells at 24 and 48 h after 5-FU treatment. Migration was measured at 12 and 24 h after razor scraping of the cell monolayer. Cell death was measured by quantifying the percentage of apoptotic and necrotic figures by flow cytometry at 12 and 24 h following 5-FU challenge. Neither glutamine nor alanyl-glutamine prevented 5-FU-induced apoptosis and necrosis in IEC-6 cells at 12 and 24 h after 5-FU challenge. However, glutamine and alanyl-glutamine enhanced migration and proliferation when compared with 5-FU-treated controls (< 0.05). These new findings support our earlier study on the benefit of oral glutamine in enhancing epithelial recovery after 5-FU challenge.

Keywords

Alanyl-glutamine Glutamine 5-fluorouracil Mucositis Chemotherapy Small intestine 

Notes

Acknowledgment

This study was supported by the following grants from the National Institutes of Health: ABC Grant no. 5 D43 TW001136, and GIDRT Grant no. 5 D43 TW006578.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Manuel B. Braga-Neto
    • 1
  • Cirle A. Warren
    • 2
  • Reinaldo B. Oriá
    • 3
    • 1
  • Manuel S. Monteiro
    • 1
  • Andressa A. S. Maciel
    • 1
  • Gerly A. C. Brito
    • 3
    • 4
  • Aldo A. M. Lima
    • 4
    • 1
  • Richard L. Guerrant
    • 1
    • 2
  1. 1.Institute of Biomedicine and Clinical Research Unit-University HospitalFederal University of CearáFortalezaBrazil
  2. 2.Center for Global Health, School of Medicine, Division of Infectious Diseases and International HealthCharlottesvilleUSA
  3. 3.Department of MorphologyFederal University of CearáFortalezaBrazil
  4. 4.Department of PharmacologyFederal University of Ceará FortalezaBrazil

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