Visceral and Somatic Hypersensitivity in TNBS-Induced Colitis in Rats
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Inflammation of visceral structures in rats has been shown to produce visceral/somatic hyperalgesia. Our objectives were to determine if trinitrobenzene sulfonic acid (TNBS) induced colitis in rats leads to visceral/somatic hypersensitivity. Male Sprague-Dawley rats (200–250 g) were treated with 20 mg of TNBS in 50% ethanol (n = 40) or an equivalent volume of ethanol (n = 40) or saline (n = 25) via the colon. Colonic distension, Von Frey, Hargreaves, and tail reflex tests were used to evaluate for visceral, mechanical, and thermal sensitivity. The rats demonstrated visceral hypersensitivity at 2–28 days following TNBS administration (P < 0.0001). The ethanol-treated rats also demonstrated visceral hypersensitivity that resolved after day 14. TNBS-treated rats demonstrated somatic hypersensitivity at days 14–28 (P < 0.0001) in response to somatic stimuli of the hind paw. TNBS colitis is associated with visceral and somatic hypersensitivity in areas of somatotopic overlap. This model of colitis should allow further investigation into the mechanisms of visceral and somatic hypersensitivity.
KeywordsTrinitrobenzene sulfonic acid induced colitis Visceral hypersensitivity Somatic hypersensitivity Viscerosomatic convergence Irritable bowel syndrome
G.N.V. is supported by a Merit Review Award (PI: GN Verne) from the Medical Research Service of the Department of Veteran Affairs and NIH Grant 1-R01-NS053090–01 (PI: GN Verne).
- 8.de Groat WC, Booth AM, Yoshimura N (1993a) Neurophysiology of micturition and its modification in animal models of human disease. In: Maggi CA (ed) The autonomic nervous system. Harwood, London, pp 227–290Google Scholar
- 9.de Groat WC, Roppolo JR, Yoshimura N, Sugaya K (1993) Neural control of the urinary bladder and colon. In: Tache Y, Wingate D, Burks T (eds) Proceedings of the second international symposium on brain-gut interactions. CRC, Boca Raton, pp 167–190Google Scholar
- 23.Tarrerias AL, Millecamps M, Alloui A, Beaughard C, Kemeny JL, Bourdu S, Bommelaer G, Eschalier A, Dapoigny M, Ardid D (2002) Short-chain fatty acid enemas fail to decrease colonic hypersensitivity and inflammation in TNBS-induced colonic inflammation in rats. Pain 100:91–97PubMedCrossRefGoogle Scholar
- 35.Zhou QQ, Caudle MR, Price DD, Del Valle-Pinero AY, Verne GN (2006) Selective up-regulation of NMDA-NR1 receptor expression in myenteric plexus after TNBS induced colitis in rats. Mol Pain 17:2–3Google Scholar