Digestive Diseases and Sciences

, Volume 53, Issue 2, pp 429–435 | Cite as

Visceral and Somatic Hypersensitivity in TNBS-Induced Colitis in Rats

  • QiQi Zhou
  • Donald D. Price
  • Robert M. Caudle
  • G. Nicholas Verne
Original Paper


Inflammation of visceral structures in rats has been shown to produce visceral/somatic hyperalgesia. Our objectives were to determine if trinitrobenzene sulfonic acid (TNBS) induced colitis in rats leads to visceral/somatic hypersensitivity. Male Sprague-Dawley rats (200–250 g) were treated with 20 mg of TNBS in 50% ethanol (n = 40) or an equivalent volume of ethanol (n = 40) or saline (n = 25) via the colon. Colonic distension, Von Frey, Hargreaves, and tail reflex tests were used to evaluate for visceral, mechanical, and thermal sensitivity. The rats demonstrated visceral hypersensitivity at 2–28 days following TNBS administration (P < 0.0001). The ethanol-treated rats also demonstrated visceral hypersensitivity that resolved after day 14. TNBS-treated rats demonstrated somatic hypersensitivity at days 14–28 (P < 0.0001) in response to somatic stimuli of the hind paw. TNBS colitis is associated with visceral and somatic hypersensitivity in areas of somatotopic overlap. This model of colitis should allow further investigation into the mechanisms of visceral and somatic hypersensitivity.


Trinitrobenzene sulfonic acid induced colitis Visceral hypersensitivity Somatic hypersensitivity Viscerosomatic convergence Irritable bowel syndrome 



G.N.V. is supported by a Merit Review Award (PI: GN Verne) from the Medical Research Service of the Department of Veteran Affairs and NIH Grant 1-R01-NS053090–01 (PI: GN Verne).


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • QiQi Zhou
    • 1
  • Donald D. Price
    • 2
    • 3
  • Robert M. Caudle
    • 2
    • 3
  • G. Nicholas Verne
    • 1
    • 4
  1. 1.Division of Gastroenterology, Hepatology, and Nutrition, Department of MedicineUniversity of Florida College of MedicineGainesvilleUSA
  2. 2.Department of Oral and Maxillofacial SurgeryUniversity of Florida Colleges of Medicine and DentistryGainesvilleUSA
  3. 3.Department of NeuroscienceUniversity of Florida Colleges of Medicine and DentistryGainesvilleUSA
  4. 4.North Florida/South Georgia VA Medical SystemGainesvilleUSA

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