Digestive Diseases and Sciences

, Volume 53, Issue 1, pp 65–72

Proteome Analysis of Human Pancreatic Ductal Adenocarcinoma Tissue Using Two-Dimensional Gel Electrophoresis and Tandem Mass Spectrometry for Identification of Disease-Related Proteins

  • Rui Tian
  • Li-Ming Wei
  • Ren-Yi Qin
  • Yan Li
  • Zhi-Yong Du
  • Wei Xia
  • Cheng-Jian Shi
  • Hong Jin
Original Paper

DOI: 10.1007/s10620-007-9823-3

Cite this article as:
Tian, R., Wei, LM., Qin, RY. et al. Dig Dis Sci (2008) 53: 65. doi:10.1007/s10620-007-9823-3

Abstract

A comparative proteomic approach has been used to identify and analyze proteins related to pancreatic cancer. Proteomes of eight pairs of clinical pancreatic ductal adenocarcinoma (PDAC) tissue samples and samples of normal adjacent tissue were obtained by two-dimensional gel electrophoresis (2DE). Comprehensive analysis of proteins was focused on total protein spots for which there were statistical differences between the two groups. Proteins were identified by peptide mass fingerprinting with tandem mass spectrometry (MS–MS). Western blotting and immunohistochemistry (IHC) were also performed to verify the expression of some candidate proteins. Thirty protein spots were identified, including proteases, antioxidant proteins, signal-transduction proteins, calcium-binding proteins, structural proteins, chaperones, and others. Western blotting and IHC confirmed up-regulated expression of two candidate proteins, nucleotide diphosphatase kinase (NDPK) and annexin II, in tumorous tissues. These results suggest that combination of 2DE with MS is an effective strategy for discovery of differently expressed proteins in PDAC which may be molecular markers for diagnosis or therapeutic targets.

Keywords

Pancreatic cancer Proteome Two-dimensional gel electrophoresis Mass spectrometry 

Abbreviations

PDAC

Pancreatic ductal adenocarcinoma

NDPK

Nucleotide diphosphatase kinase

IHC

Immunohistochemistry

IAA

Iodoacetamide

CHAPS

3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulfonate

DTT

Dithiothuretiol

ACN

Acetonitrile

TFA

Trifluoroacetic acid

CHCA

α-Cyano-4-hydroxycinnamic acid

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Rui Tian
    • 1
  • Li-Ming Wei
    • 2
  • Ren-Yi Qin
    • 1
  • Yan Li
    • 3
  • Zhi-Yong Du
    • 1
  • Wei Xia
    • 1
  • Cheng-Jian Shi
    • 1
  • Hong Jin
    • 4
  1. 1.Department of Biliary-Pancreatic SurgeryAffiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyWuhanP.R. China
  2. 2.Institutes of Biomedical SciencesFudan UniversityShanghaiP.R. China
  3. 3.Department of DermatologyAffiliated Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyWuhanP.R. China
  4. 4.Department of ChemistryFudan UniversityShanghaiP.R. China

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