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Digestive Diseases and Sciences

, Volume 52, Issue 9, pp 2292–2300 | Cite as

Stem Cell Factor/c-kit Receptor Signaling Enhances the Proliferation and Invasion of Colorectal Cancer Cells Through the PI3K/Akt Pathway

  • Akira YasudaEmail author
  • Hirozumi Sawai
  • Hiroki Takahashi
  • Nobuo Ochi
  • Yoichi Matsuo
  • Hitoshi Funahashi
  • Mikinori Sato
  • Yuji Okada
  • Hiromitsu Takeyama
  • Tadao Manabe
Original Paper

Abstract

In this study, we examined the role of c-kit receptor (KIT) signal transduction on the proliferation and invasion of colorectal cancer cells. We found that c-kit was expressed in 2 colorectal cancer cell lines as determined by RT-PCR, Western blot, and flow cytometry. In KIT-positive lines, KIT was activated by stem cell factor (SCF). SCF enhanced cellular proliferation of positive lines as demonstrated by the WST-1 proliferation assay. Furthermore, SCF enhanced the invasive ability of KIT-positive cell lines. SCF stimulation upregulated p44/42 mitogen-activated protein kinase (MAPK) and Akt as shown by Western blot. We examined the roles played by p44/42 MAPK and phosphatidylinositol 3-kinase (PI3K)/Akt pathways in proliferation and invasion. PI3K/Akt activity strongly correlated with proliferation and invasion and p44/42 MAPK was correlated with only invasion. In conclusion, the SCF-enhanced proliferation and invasion of KIT-positive colorectal cancer cells is achieved mainly through the PI3K/Akt pathway.

Keywords

c-kit SCF Colorectal cancer PI3K/Akt Proliferation Invasion 

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Akira Yasuda
    • 1
    Email author
  • Hirozumi Sawai
    • 1
  • Hiroki Takahashi
    • 1
  • Nobuo Ochi
    • 1
  • Yoichi Matsuo
    • 1
  • Hitoshi Funahashi
    • 1
  • Mikinori Sato
    • 1
  • Yuji Okada
    • 1
  • Hiromitsu Takeyama
    • 1
  • Tadao Manabe
    • 1
  1. 1.Department of Gastroenterological SurgeryNagoya City University Graduate School of Medical SciencesNagoyaJapan

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