Digestive Diseases and Sciences

, Volume 53, Issue 10, pp 2697–2701 | Cite as

Immunoreactivity of Antibodies Against Transglutaminase-Deamidated Gliadins in Adult Celiac Disease

  • Maria Letizia Falini
  • Luca Elli
  • Rosita Caramanico
  • Maria Teresa Bardella
  • Claudia Terrani
  • Leda Roncoroni
  • Luisa Doneda
  • Fabio Forlani
Original Paper
First Online:
Received:
Accepted:

Abstract

Background The significance of the presence of anti-gliadin antibodies in patients affected by celiac disease is still unclear. It is hypothesized that gliadin deamidation, catalysed by transglutaminase, plays a role in favoring the antigen presentation. Aim To determine the immunoreactivity of anti-gliadin antibodies from untreated celiac patients to transglutaminase deamidated gliadins. Materials and methods Gliadins from wheat flour underwent enzymatic digestion and were deamidated or cysteamine-transamidated by transglutaminase. Immunoreactivity of anti-gliadin antibodies from untreated adult celiac patients sera was evaluated by means of a competitive enzyme-linked immunosorbent assay (ELISA) method. Results Gliadin deamidation increased antibodies immunoreactivity from 25% to 50% while cysteamine incorporation into the gliadin peptides resulted in an immunoreactivity decrease. Conclusions Increased immunoreactivity of transglutaminase deamidated gliadins tested with anti-gliadin antibodies from untreated adult celiac patients supports the hypothesis of a pivotal role of gliadin deamidation in the pathomechanism of celiac disease.

Keywords

Celiac disease Gliadin Anti-gliadin antibodies Transglutaminase 
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Notes

Acknowledgements

The authors particularly thank the Lombardy Section of the Italian Association for Celiac Disease for its support.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Maria Letizia Falini
    • 1
  • Luca Elli
    • 2
    • 3
  • Rosita Caramanico
    • 1
  • Maria Teresa Bardella
    • 2
  • Claudia Terrani
    • 2
  • Leda Roncoroni
    • 2
    • 3
  • Luisa Doneda
    • 4
  • Fabio Forlani
    • 1
  1. 1.Department of Agrifood Molecular ScienceUniversity of MilanMilanItaly
  2. 2.Center for the Prevention and Diagnosis of Celiac DiseaseFondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina ElenaMilanoItaly
  3. 3.Department of Medical SciencesUniversity of MilanMilanItaly
  4. 4.Department of Biology and Genetic for Medical SciencesUniversity of MilanMilanItaly

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