Digestive Diseases and Sciences

, Volume 53, Issue 7, pp 1917–1928

Dendritic Cells and Toll-Like Receptors 2 and 4 in the Ileum of Crohn's Disease Patients

  • Manuel A. Silva
  • Rodrigo Quera
  • Jorge Valenzuela
  • Sa’ad Y. Salim
  • Johan D. Söderholm
  • Mary H. Perdue
Original Paper

DOI: 10.1007/s10620-007-0105-x

Cite this article as:
Silva, M.A., Quera, R., Valenzuela, J. et al. Dig Dis Sci (2008) 53: 1917. doi:10.1007/s10620-007-0105-x

Abstract

We investigated myeloid-dendritic cell (DC) marker and Toll-like receptor (TLR)-2 and 4 distributions in ileal samples from Crohn's disease (CD) patients (n = 14) and controls (n = 13). In controls, no TLR-2+ cells were observed, and higher numbers of TLR-4+ and DC-SIGN+ cells (P < 0.01) were detected in ileal samples when compared versus colonic tissues. In non-inflamed CD ileum, TLR-4+ and DC-SGN+ cells were depleted from superficial areas of the villus, and a significant CD1a+ cell infiltration (P < 0.01) was observed when compared to ileal controls and non-inflamed colonic CD samples. In inflamed CD ileum, DC-SIGN+, CD1a+, TLR-4+ and few TLR-4+DC-SIGN+ cells were detected as well as CD83 depletion. No correlation between TLR-2 and DC markers was detected in CD samples. A unique distribution of myeloid-DC markers characterized the CD ileum. Also, the presence of significant amounts of ileal CD1a+ cells may provide a relevant DC-mediated mechanism for antigen recognition in the pathogenesis of CD.

Keywords

Crohn's disease Dendritic cells Inflammation Toll-like receptor CD1a 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Manuel A. Silva
    • 1
  • Rodrigo Quera
    • 2
  • Jorge Valenzuela
    • 2
  • Sa’ad Y. Salim
    • 3
  • Johan D. Söderholm
    • 3
  • Mary H. Perdue
    • 1
  1. 1.Intestinal Disease Research Programme, Department of Pathology and Molecular MedicineMcMaster UniversityHamiltonCanada
  2. 2.Gastroenterology Division, Internal Medicine DepartmentUniversity of Chile Clinical Hospital and Clínica Las CondesSantiagoChile
  3. 3.Department of Biomedicine and Surgery, Division of SurgeryUniversity HospitalLinköpingSweden

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