Crohn’s Disease and SLC11A1 Promoter Polymorphism
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Crohn’s disease (CD) is a chronic multifactorial inflammatory disease. The prevalence of CD in Ashkenazi Jews is higher than in Sephardic Jews. SLC11A1, also known as Nramp1, is a divalent cation antiporter essential for the elimination of intraphagosomal pathogens. SLC11A1 has seven alleles in the promoter region and previous studies have suggested an association between CD and SLC11A1. The aim of this study was to check for a possible association between SLC11A1 promoter alleles and CD in Ashkenazi Jewish patients. DNA samples from healthy Ashkenazi donors and Ashkenazi CD patients were obtained and analyzed for SLC11A1 promoter polymorphism by PCR and DNA sequencing. One hundred thirty-one samples from healthy donors and 131 samples from CD patients were analyzed. Four alleles were identified: ∼70% of the samples carried allele 3; ∼30%, allele 2; ∼1%, allele 1; and <1%, allele 5. There was no difference in allele frequencies between healthy donors and CD patients. No correlation was found between mutations in NOD2/CARD15 and the phenotype of CD. We conclude that the difference in SLC11A1 promoter polymorphism plays no role in CD in Ashkenazi Jews.
KeywordsCrohn’s disease SLC11A1 NRAMP Promoter polymorphism Alleles Genetics Ulcerative colitis Inflammatory bowel disease
We are grateful to Drs. Darvasi and Yakir of the Hebrew University in Jerusalem for their help with statistical analysis. This research was supported by the Broad Medical Research Program of The Eli and Edythe L. Broad Foundation to B.Z.L. and R.E.
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