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Digestive Diseases and Sciences

, Volume 51, Issue 12, pp 2257–2263 | Cite as

Pretreatment of Hypertonic Saline Can Increase Endogenous Interleukin 10 Release to Attenuate Hepatic Ischemia Reperfusion Injury

  • Qing-Hong Ke
  • Shu-Sen Zheng
  • Ting-Bo Liang
  • Hai-Yang Xie
  • Wei-Liang Xia
Original Paper

Abstract

Ischemia–reperfusion (I/R) injury of the liver occurs in many clinical cases. Many steps are associated with hepatic I/R injury, including the release of many inflammatory molecules and infiltration of neutrophils into the liver. Recent studies revealed that hypertonic saline (HTS) has a strong anti-inflammatory effect and can inhibit a varity of neutrophil functions. So pretreatment with HTS may attenuate the liver injury associated with I/R. In this study, rats were divided into three groups: the sham group (S group), hepatic I/R group (I/R group), and HTS pretreatment group (HTS group). Serum ALT and myeloperoxidase (MPO) activity were determined. Serum tumor necrosis factor α (TNF-α) and interleukin 10 (IL-10) were determined by enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction analysis was used to assess the mRNA expressions of TNF-α and IL-10. Protein expressions of TNF-α, IL-10, STAT3, and phosphorylated STAT3 were analyzed by Western blot. Results showed that HTS pretreatment can augment the release of endogenous IL-10 by activating STAT3 in the process of hepatic I/R injury. Serum ALT levels, MPO activity in liver, generation of TNF-α, and infiltration of neutrophils in liver were inhibited in the HTS group. So we concluded that HTS pretreatment attenuates hepatic I/R injury by increasing the release of endogenous IL-10.

Keywords

Ischemia–reperfusion Liver Hypertonic saline Interleukin 10 Prevention 

Notes

Acknowledgment

This study was supported by a research grant from the National Natural Science Foundation of China (No. 30371358).

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Qing-Hong Ke
    • 1
  • Shu-Sen Zheng
    • 1
  • Ting-Bo Liang
    • 1
  • Hai-Yang Xie
    • 1
  • Wei-Liang Xia
    • 1
  1. 1.Department of Hepatobiliary and Pancreatic Surgery The First Affiliated Hospital, College of Medicine, Key Lab of Combined Muti-organ Transplantation, Ministry of HealthZhejiang UniversityHangzhouP.R. China

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