Intestinal Damage in Strongyloidiasis: The Imbalance Between Cell Death and Proliferation

  • Ana Luiza Werneck-Silva
  • Eliana Parisi Alvares
  • Patrícia Gama
  • Aderson Omar Mourão Cintra Damião
  • Luciana Harumi Osaki
  • Daniela Ogias
  • Aytan Miranda Sipahi
Intestinal Disease

Abstract

Strongyloidiasis is an endemic tropical parasitosis caused by Strongyloides stercoralis that also affects immigrants in nontropical countries. The nematode colonizes the duodenum and upper jejunum, inducing mucosal alterations. Because integrity is essential for a functional barrier, we aimed to study apoptosis and proliferation in the small bowel epithelium infected with S. stercoralis. We evaluated 23 patients and 17 controls. Apoptotic cells were detected by TUNEL and M30 immunolabelling, whereas proliferation was scored by Ki67 immunostaining and mitotic counting. Infection increased apoptotic indices in duodenum and jejunum (P < 0.001). Conversely, it decreased cell proliferation in both segments (P < 0.001). Our results showed that intestinal strongyloidiasis promotes an imbalance between cell death and proliferation. This is the first evidence of disruption of the epithelial kinetics with S. stercoralis infection, though the mechanisms remain unclear. Furthermore, our results support the idea that strongyloidiasis disturbs the mucosal integrity and can compromise the intestinal barrier.

Keywords

strongyloidiasis small intestine apoptosis cell proliferation 

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Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • Ana Luiza Werneck-Silva
    • 1
  • Eliana Parisi Alvares
    • 2
    • 3
  • Patrícia Gama
    • 2
  • Aderson Omar Mourão Cintra Damião
    • 1
  • Luciana Harumi Osaki
    • 2
  • Daniela Ogias
    • 2
  • Aytan Miranda Sipahi
    • 1
  1. 1.Department of Gastroenterology, School of Medicine, Laboratory of Investigation (LIM 07)University of São PauloSão PauloBrazil
  2. 2.Department of Cell and Developmental Biology, Institute of Biomedical SciencesUniversity of São PauloSão PauloBrazil
  3. 3.Av Prof Lineu Prestes 1524 ICB ISão PauloBrazil

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