Digestive Diseases and Sciences

, Volume 51, Issue 3, pp 548–559

Rat Gastric Gelatinase Induction During Endotoxemia

  • Emily K. Robinson
  • Sonlee D. West
  • Alian Garay
  • DAVID W. MERCER
Esophageal, Gastric, and Duodenal Disorders

DOI: 10.1007/s10620-006-3169-0

Cite this article as:
Robinson, E.K., West, S.D., Garay, A. et al. Dig Dis Sci (2006) 51: 548. doi:10.1007/s10620-006-3169-0

Abstract

Despite continued investigation, the pathogenesis of tissue injury secondary to sepsis remains elusive. Further evaluation of the mechanisms by which endotoxemia and sepsis induce tissue injury is necessary to formulate rational and effective treatment strategies. The purpose of these studies was to evaluate the role of the matrix metalloproteinases MMP-2 and MMP-9 in gastric injury during lipopolysaccharide induced endotoxemia. Lipopolysaccharide increased gastric gelatinase activity as determined by in situ and gelatin zymography. Specifically, lipopolysaccharide induced MMP-2, MMP-9, and tissue inhibitor of metalloproteinase-1 (TIMP-1) transcription, with subsequent increases in MMP-2 and TIMP-1 protein expression. Furthermore, selective metalloproteinase inhibition ameliorated gastric injury in this model. These data suggest that lipopolysaccharide-induced gastric injury is mediated, at least in part, by increased MMP-2 production.

Key Words

gelatinase zymography tissue inhibitor of metalloproteinase (TIMP) lipopolysaccharide matrix metalloproteinase 

Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • Emily K. Robinson
    • 1
    • 2
  • Sonlee D. West
    • 1
  • Alian Garay
    • 1
  • DAVID W. MERCER
    • 1
  1. 1.Department of SurgeryThe University of Texas Health Science Center at HoustonHoustonUSA
  2. 2.UT-Houston Medical School/LBJ General HospitalHoustonUSA

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