Digestive Diseases and Sciences

, Volume 51, Issue 11, pp 1934–1941

Allergen-Specific In Vitro Cytokine Production in Adult Patients with Eosinophilic Esophagitis

  • Kiyoshi Yamazaki
  • Joseph A. Murray
  • Amindra S. Arora
  • Jeffery A. Alexander
  • Thomas C. Smyrk
  • Joseph H. Butterfield
  • Hirohito Kita
Original Paper

DOI: 10.1007/s10620-005-9048-2

Cite this article as:
Yamazaki, K., Murray, J.A., Arora, A.S. et al. Dig Dis Sci (2006) 51: 1934. doi:10.1007/s10620-005-9048-2

Abstract

Although the pathogenesis of eosinophilic esophagitis (EE) likely involves hypersensitivity reactions against exogenous allergens, allergen-specific cellular immune responses have not been studied. We investigated allergen-induced cytokine production by peripheral blood mononuclear cells (PBMCs) in adult patients with EE (n=15) and healthy controls (HC; n=9). PBMCs were incubated with nine common food and environmental allergens or a nonspecific mitogen, and the levels of interleukin (IL)-5, IL-10, IL-13, and interferon-γ in the cell-free supernatants were determined. Spontaneous and mitogen-stimulated cytokine production did not differ between EE and HC. House dust mite, ragweed, Aspergillus, milk, and soy induced significantly higher IL-5 production in EE (P < 0.05). House dust mite also augmented IL-13 production in EE (P < 0.05). Furthermore, PBMCs from three EE patients without allergen-specific IgE vigorously produced IL-5 and IL-13 on allergen stimulation. Thus, immune responses in EE are characterized by enhanced production of Th2-like cytokines against both food and environmental allergens.

Keywords

Eosinophils Esophagitis Interleukin-5 Interleukin-13 Allergens Immunoglobulin E 

Copyright information

© Springer Science&#x0002B;Business Media, Inc. 2006

Authors and Affiliations

  • Kiyoshi Yamazaki
    • 1
    • 5
  • Joseph A. Murray
    • 2
  • Amindra S. Arora
    • 2
  • Jeffery A. Alexander
    • 2
  • Thomas C. Smyrk
    • 3
  • Joseph H. Butterfield
    • 1
  • Hirohito Kita
    • 1
    • 4
  1. 1.Division of Allergic Diseases, Department of Internal MedicineMayo Clinic College of MedicineRochesterUSA
  2. 2.Division of Gastroenterology and Hepatology, Department of Internal MedicineMayo Clinic College of MedicineRochesterUSA
  3. 3.Division of Anatomic Pathology, Department of Medicine and PathologyMayo Clinic College of MedicineRochesterUSA
  4. 4.Allergic Diseases Research Laboratory, Division of Allergic Diseases, Department of Internal MedicineMayo Clinic College of MedicineRochesterUSA
  5. 5.Sanyudo HospitalYonezawaJapan

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