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Digestive Diseases and Sciences

, Volume 50, Issue 7, pp 1361–1371 | Cite as

Hepatitis C Virus Core Protein Modulates Fatty Acid Metabolism and Thereby Causes Lipid Accumulation in the Liver

  • Atsushi Yamaguchi
  • Susumu TazumaEmail author
  • Tomoji Nishioka
  • Waka Ohishi
  • Hideyuki Hyogo
  • Shuichi Nomura
  • Kazuaki Chayama
Article

Abstract

We studied the roles of hepatitis C virus (HCV) core protein in hepatic steatosis and changes in hepatic lipid metabolism. HCV core protein expression plasmid was transfected in HepG2 . Triacylglyceride (TG) and mRNA level associated with lipid metabolism were measured. Male C57BL/6 mice were infected with HCV core recombinant adenovirus and used for lipids and mRNA studies. In HCV core protein-expressing cells, peroxisome proliferator-activated receptor (PPAR)α, multidrug resistance protein (MDR) 3, and microsomal triglyceride transfer protein (MTP) were down-regulated 48 hr after transfection. In HCV core protein-expressing mice, hepatic TG content and hepatic thiobarbituric acid-reactive substances increased. PPARα, MDR2, acyl-CoA oxidase (AOX), and carnitine palmitoyl transferase-1 (CPT-1) were down-regulated. HCV core protein down-regulated lipid metabolism-associated gene expression, Mdr2, CPT, and AOX, accompanied by down-regulation of PPARα. There findings may contribute to the understanding of HCV-related steatosis, induction of reactive oxygen species, and carcinogenesis.

Key Words

HCV core protein steatosis nuclear receptor ABC transporter 

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References

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    Moriya K, Fujie H, Shintani Y, Yotsuyanagi H, Tsutsumi T, Ishibashi K, Matsuura Y, Kimura S, Miyamura T, Koike K: The core protein of hepatitis C virus induces hepatocellular carcinoma in transgenic mouse. Nat Med 4:1065–1067, 1998PubMedGoogle Scholar
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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Atsushi Yamaguchi
    • 1
  • Susumu Tazuma
    • 2
    • 3
    Email author
  • Tomoji Nishioka
    • 1
  • Waka Ohishi
    • 1
  • Hideyuki Hyogo
    • 2
  • Shuichi Nomura
    • 2
  • Kazuaki Chayama
    • 1
  1. 1.Departments of Medicine and Molecular ScienceGraduate School of Biomedical Sciences, Hiroshima UniversityHiroshimaJapan
  2. 2.Departments of General Medicine and Clinical PharmacotherapyGraduate School of Biomedical Sciences, Hiroshima UniversityHiroshimaJapan
  3. 3.Department of General Medicine and Clinical PharmacotherapyGraduate School of Biomedical Sciences, Hiroshima UniversityHiroshimaJapan

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