Digestive Diseases and Sciences

, Volume 50, Issue 1, pp 181–187

Expression of Fc Fragment Receptors of Immunoglobulin G (FcγRs) in Rat Hepatic Stellate Cells

  • Hong Shen
  • Manna Zhang
  • Kelly Kaita
  • Gerald Y. Minuk
  • Julia Rempel
  • Yuewen Gong
Article

DOI: 10.1007/s10620-005-1298-5

Cite this article as:
Shen, H., Zhang, M., Kaita, K. et al. Dig Dis Sci (2005) 50: 181. doi:10.1007/s10620-005-1298-5

Hepatic stellate cells (HSCs) are now considered the major cell type in the liver mediating the development of liver fibrosis. Recently it was demonstrated that HSCs express membrane proteins involved in antigen presentation. We further evaluate immunological properties of HSCs by examining the expression and function of the Fc fragment of immunoglobulin G (IgG) in HSCs. In this study, we document the presence of mRNAs for three FcγRs in HSCs. Ligand binding assay indicated the existence of FcγRs with different binding affinities on membranes of HSCs. We also documented that the abundance of the three Fcγ R mRNAs increased upon activation of HSCs in vitro. Moreover, an examination of the biological activities of IgG revealed that exposure to IgG significantly stimulated HSC differentiation and proliferation. Furthermore, we studied the intracellular signaling protein, LcK, in HSCs and regulation of Lck expression and phosphorylation by IgG. Although IgG did not regulate Lck abundance and phosphorylation in HSCs, highly phosphorylated Lck was present in these cells. In conclusion, we provided evidence that HSCs expresses receptors for the Fc fragment of IgG, and IgG regulates HSC differentiation and proliferation. Therefore, immunoglobulin G may play a role in HSC activation.

KEY WORDS:

Fc fragment receptors immunoglobulin G expression hepatic stellate cells 

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Hong Shen
    • 1
    • 2
  • Manna Zhang
    • 2
  • Kelly Kaita
    • 2
  • Gerald Y. Minuk
    • 2
  • Julia Rempel
    • 2
  • Yuewen Gong
    • 1
    • 2
  1. 1.Faculty of PharmacyUniversity of ManitobaWinnipegCanada
  2. 2.Departments of Internal Medicine, Faculty of MedicineUniversity of ManitobaWinnipegCanada
  3. 3.Faculty of PharmacyUniversity of ManitobaWinnipegCanada R3T 2N2

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