, Volume 71, Issue 6, pp 1095–1108 | Cite as

Antitumor effects of seleno-short-chain chitosan (SSCC) against human gastric cancer BGC-823 cells

  • Xiao-dan Dong
  • Juan Yu
  • Fan-qi Meng
  • Ying-ying Feng
  • Hai-yu Ji
  • Anjun LiuEmail author
Original Article


Seleno-short-chain chitosan (SSCC) is a derivative of chitosan. In the present study, we sought to investigate the underlying antitumor mechanism of SSCC on human gastric cancer BGC-823 cells in vitro. MTT assay suggested that SSCC exhibited a dose-dependent inhibitory effect on the proliferation of BGC-823 cells. We found the SSCC-treated cells showed typical morphological characteristics of apoptosis in a dose dependent manner by observing on microscope. Annexin V-FITC/PI double staining and cell cycle assay identified that SSCC could induce BGC-823 cells apoptosis by triggering G2/M phase arrest. Our research provided the first evidence that SSCC could effectively induce the apoptosis of BGC-823 cells via an intrinsic mitochondrial pathway, as indicated by inducing the disruption of mitochondrial membrane potential (MMP), the excessive accumulation of reactive oxidative species (ROS), the increase of Bax/Bcl-2 ratio and the activation of caspase 3, caspase 9 and cytochrome C (Cyt-C) in BGC-823 cells. These combined results clearly indicated that SSCC could induce BGC-823 cells apoptosis by the involvement of mitochondrial signaling pathway, which provided precise experimental evidence for SSCC as a potential agent in the prevention and treatment of human gastric cancer.


SSCC Human gastric cancer Cell apoptosis Mitochondrial pathway 


Compliance with ethical standards

Conflicts of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Xiao-dan Dong
    • 1
    • 2
  • Juan Yu
    • 1
    • 2
  • Fan-qi Meng
    • 1
    • 2
  • Ying-ying Feng
    • 1
    • 2
  • Hai-yu Ji
    • 1
    • 2
  • Anjun Liu
    • 1
    • 3
    Email author
  1. 1.Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and BiotechnologyTianjin University of Science and TechnologyTianjinChina
  2. 2.QingYunTang Biotech (Beijing) Co., LtdBeijingChina
  3. 3.Tianjin Key Laboratory of Food Nutrition and Safety, School of Food Engineering and Biological TechnologyTianjin University of Science and TechnologyTianjinChina

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