, Volume 71, Issue 5, pp 1003–1017 | Cite as

Sambucus nigra L. ameliorates UVB-induced photoaging and inflammatory response in human skin keratinocytes

  • Pei Lin
  • Eunson Hwang
  • Hien T. T. Ngo
  • Seul A. Seo
  • Tae-Hoo YiEmail author
Original Article


Sambucus nigra L. (Elderberry) is widely used as a dietary supplement in functional food and possesses many pharmacological activities to prevent ailments, such as the colds and fever, diabetes and cancer. However, research on its skin anti-aging effect is still limited. Here, we evaluated the recovery effects of elderberry extract (EB) in UVB-irradiated human skin keratinocytes (HaCaTs) and investigated whether EB represents a potential therapeutic agent against skin photoaging and inflammation. In this study, EB showed good efficiency on scavenging free radicals and dose-dependently reduced reactive oxygen species (ROS) generation. EB notably decreased UVB-induced matrix metalloproteinase-1 (MMP-1) expression and inflammatory cytokine secretion through the inhibition of mitogen-activated protein kinases/activator protein 1 (MAPK/AP-1) and nuclear factor-κB (NF-κB) signaling pathways, blocking extracellular matrix (ECM) degradation and inflammation in UVB-irradiated HaCaTs. In addition, EB improved nuclear factor E2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) signaling to increase oxidative defense capacity, and enhanced transforming growth factor beta (TGF-β) signaling activation to promote procollagen type I synthesis, relieving UVB-induced skin cell damage. These results indicated that EB has the potential to ameliorate UVB-induced skin photoaging and inflammation.


Elderberry UVB irradiation HaCaTs Photoaging ROS MMP-1 



This work was supported by the Snow White Factory Inc., Republic of Korea.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.College of Life ScienceKyung Hee UniversityYongin-siRepublic of Korea

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