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Cytotechnology

, Volume 71, Issue 2, pp 635–646 | Cite as

Vildagliptine protects SH-SY5Y human neuron-like cells from Aβ 1–42 induced toxicity, in vitro

  • Alim Hüseyin DokumacıEmail author
  • Mukerrem Betul Yerer Aycan
Article

Abstract

The amyloid β (Aβ) toxic fibrils is thought to play a central role in the onset and progression of Alzheimer’s disease (AD) because of it is a main formation of senile plaques. Diabetic patients are more vulnerable to caught Alzheimer’s disease. Vildagliptine, a novel anti diabetic agent, has been reported to exert protective effects on AD rat models in restricted study. We aimed to investigate any protective effects of vildagliptine against Aβ fibrils on SH-SY5Y cell line. Vildagliptine decreased PSEN1 and PSEN2 mRNA levels which enroll Aβ production. In addition, vildagliptin was downregulated caspase-3 and caspase-9 expression levels which were evoked by Aβ. Also we confirmed cellular viability with real time cell analyzer and MTT assay. Our data exposed that vildagliptine has lowering effect on GSK3β and Tau phosphorylation. However we did not get protective effect of vildagliptine against Aβ toxicity on mitochondrial membrane potential. These results indicate that vildagliptine exerts a protective effect against Aβ by decreasing apoptosis related proteins, lowering GSK3β and Tau phosphorylation levels in addition to expression of PSEN1 and PSEN2 mRNA downregulation effect.

Keywords

Cell viability Vildagliptine Amyloid beta Apoptosis Memantine 

Abbreviations

AD

Alzheimer’s disease

Aβ

Amyloid beta

Vilda

Vildagliptine

Mem

Memantine

GSK3β

Glycogen synthase kinase 3 beta

APP

Amyloid precursor protein

PSEN1

Presenilin 1

PSEN2

Presenilin2

Notes

Acknowledgements

Funding was provided by Erciyes Üniversitesi (Grant No. TDK-5824).

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of PharmacologyErciyes University Faculty of PharmacyKayseriTurkey

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