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Cytotechnology

, Volume 71, Issue 1, pp 389–399 | Cite as

Sargassum sagamianum extract protects INS-1 pancreatic β cells against high glucose-induced apoptosis

  • Ji-Soo Lee
  • Hyun-Ah Lee
  • Ji⁃Sook HanEmail author
Original Article
First Online:
  • 35 Downloads

Abstract

This study investigated the protective effects of Sargassum sagamianum extract (SSE) on INS-1 pancreatic β cells against high glucose-induced oxidative stress and apoptosis. Treatment with glucose at high concentrations (30 mM) caused β cell apoptosis, whereas treatment with SSE protected the β cells from high glucose-induced damage, by recovering the cell viability. Treatment with SSE at concentrations of 10–100 μg/mL decreased lipid peroxidation and intracellular reactive oxygen species and nitric oxide levels, and increased cell viability and insulin secretion in high glucose pretreated INS-1 cells in a dose-dependent manner. Moreover, SSE treatment significantly reduced the expression of pro-apoptotic Bax, cytochrome c, caspase-3, and caspase-9, while the expression of anti-apoptotic Bcl-2 increased. The type of cell death was examined by annexin V/propidium iodide staining, which revealed that SSE treatment markedly reduced high glucose-induced apoptosis. These findings suggest that SSE could be useful as a functional food, protecting pancreatic β cells against high glucose-induced oxidative stress and apoptosis.

Keywords

Apoptosis INS-1 Pancreatic β cells Sargassum sagamianum 
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Notes

Acknowledgements

We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.

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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Food Science and NutritionPusan National UniversityBusanRepublic of Korea

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