Advertisement

Parkia speciosa empty pod extract exerts anti-inflammatory properties by modulating NFκB and MAPK pathways in cardiomyocytes exposed to tumor necrosis factor-α

  • J. S. Gui
  • J. Jalil
  • Z. Jubri
  • Y. Kamisah
Article
  • 11 Downloads

Abstract

Parkia speciosa Hassk is a plant found abundantly in the Southeast Asia region. Its seeds, with or without pods, have been used in traditional medicine locally to treat cardiovascular problems. The pathogenesis of cardiovascular diseases involves inflammation and oxidative stress. Based on this information, we sought to investigate the potential protective effects of Parkia speciosa empty pod extract (PSE) on inflammation in cardiomyocytes exposed to tumor necrosis factor-α (TNF-α). H9c2 cardiomyocytes were divided into four groups; negative control, TNF-α, PSE + TNF-α and quercetin + TNF-α. Groups 3 and 4 were pretreated with PSE ethyl acetate fraction of ethanol extract (500 µg/mL) or quercetin (1000 µM, positive control) for 1 h before inflammatory induction with TNF-α (12 ng/mL) for 24 h. TNF-α increased protein expression of nuclear factor kappa B cell (NFκB) p65, p38 mitogen-activated protein kinase (p38 MAPK), inducible nitric oxide synthase, cyclooxygenase-2 and vascular cell adhesion molecule-1 when compared to the negative control (p < 0.05). It also elevated iNOS activity, nitric oxide and reactive oxygen species levels. These increases were significantly reduced with PSE and quercetin pretreatments. The effects of PSE were comparable to that of quercetin. PSE exhibited anti-inflammatory properties against TNF-α-induced inflammation in H9c2 cardiomyocytes by modulating the NFκB and p38 MAPK pathways.

Keywords

TNF-α Inflammation Petai NFκB MAPK 

Notes

Acknowledgements

The authors would like to acknowledge the financial support from Universiti Kebangsaan Malaysia (UKM) Grant (AP-2014-013) and technical help from Puan Nurul Hafizah Abas, Encik Fadhlullah Zuhair Japar Sidik and Puan Juliana Abdul Hamid.

References

  1. Baeuerle PA, Baichwal VR (1997) NF-kappa B as a frequent target for immunosuppressive and anti-inflammatory molecules. Adv Immunol 65:111–137CrossRefGoogle Scholar
  2. Bergmann MW, Loser P, Dietz R, von Harsdorf R (2001) Effect of NF-kappa B Inhibition on TNF-alpha-induced apoptosis and downstream pathways in cardiomyocytes. J Mol Cell Cardiol 33:1223–1232CrossRefGoogle Scholar
  3. Bhaskar S, Sudhakaran PR, Helen A (2016) Quercetin attenuates atherosclerotic inflammation and adhesion molecule expression by modulating TLR-NF-κB signaling pathway. Cell Immunol 310:131–140CrossRefGoogle Scholar
  4. Boots AW, Wilms LC, Swennen EL, Kleinjans JC, Bast A, Haenen GR (2008) In vitro and ex vivo anti-inflammatory activity of quercetin in healthy volunteers. Nutrition 24:703–710CrossRefGoogle Scholar
  5. Chan ED, Riches DW (1998) Potential role of the JNK/SAPK signal transduction pathway in the induction of iNOS by TNF-alpha. Biochem Biophys Res Commun 253:790–796CrossRefGoogle Scholar
  6. Chen CC, Sun YT, Chen JJ, Chiu KT (2000) TNF-α-induced cyclooxygenase-2 expression in human lung epithelial cells: involvement of the phospholipase C-γ2, protein kinase C-α, tyrosine kinase, NF-κB-inducing kinase, and I-κB kinase 1/2 pathway. J Immunol 165:2719–2728CrossRefGoogle Scholar
  7. Chen YW, Chou HC, Lin ST, Chen YH, Chang YJ, Chen L, Chan HL (2013) Cardioprotective effects of quercetin in cardiomyocyte under ischemia/reperfusion injury. Evid Based Complement Alternat Med 2013:364519PubMedPubMedCentralGoogle Scholar
  8. Denise Martin E, De Nicola GF, Marber MS (2012) New therapeutic targets in cardiology: p38 alpha mitogen-activated protein kinase for ischemic heart disease. Circulation 126:357–368CrossRefGoogle Scholar
  9. Dhawan V (2014) Reactive oxygen and nitrogen species: general considerations. In: Saha GK, Jindal SK, Biswal S, Barnes PJ, Pawankar R (eds) Studies on respiratory disorders. Humana Press, New York, pp 27–47CrossRefGoogle Scholar
  10. García-Mediavilla V, Crespo I, Collado PS, Esteller A, Sánchez-Campos S, Tuñón MJ, González-Gallego J (2007) The anti-inflammatory flavones quercetin and kaempferol cause inhibition of inducible nitric oxide synthase, cyclooxygenase-2 and reactive C-protein, and down-regulation of the nuclear factor kappaB pathway in Chang liver cells. Eur J Pharmacol 557:221–229CrossRefGoogle Scholar
  11. Gordon JW, Shaw JA, Kirshenbaum LA (2011) Multiple facets of NF-κB in the heart: to be or not to NF-κB. Circ Res 108:1122–1132CrossRefGoogle Scholar
  12. Huang GJ, Huang SS, Deng JS (2012) Anti-inflammatory activities of inotilone from Phellinus linteus through the inhibition of MMP-9, NF-κB, and MAPK activation in vitro and in vivo. PLoS ONE 7:e35922CrossRefGoogle Scholar
  13. Kamisah Y, Othman F, Qodriyah HM, Jaarin K (2013) Parkia speciosa Hassk.: a potential phytomedicine. Evid Based Complement Alternat Med 2013:1–9CrossRefGoogle Scholar
  14. Kamisah Y, Zuhair JSF, Juliana AH, Jaarin K (2017) Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N(G)-nitro-l-arginine methyl ester. Biomed Pharmacother 96:291–298CrossRefGoogle Scholar
  15. Karunaweera N, Raju R, Gyengesi E, Münch G (2015) Plant polyphenols as inhibitors of NF-kB induced cytokine production—a potential anti-inflammatory treatment for Alzheimer’s disease? Front Mol Neurosci 8:1–5CrossRefGoogle Scholar
  16. Ko HJ, Ang LH, Ng LT (2014) Antioxidant activities and polyphenolic constituents of bitter bean Parkia speciosa. Int J Food Prop 17:1977–1986CrossRefGoogle Scholar
  17. Kyriakis JM, Avruch J (2012) Mammalian MAPK signal transduction pathways activated by stress and inflammation: a 10-year update. Physiol Rev 92:689–737CrossRefGoogle Scholar
  18. Lawrence T (2009) The nuclear factor NF-κB pathway in inflammation. Cold Spring Harb Perspect Biol 1:a001651CrossRefGoogle Scholar
  19. Li L, Wang L, Wu Z, Yao L, Wu Y, Huang L, Liu K, Zhou X, Gou D (2014) Anthocyanin-rich fractions from red raspberries attenuate inflammation in both RAW264.7 macrophages and a mouse model of colitis. Sci Rep 4:6234CrossRefGoogle Scholar
  20. Lim TK (2012) Parkia speciosa. Edible medicinal and non-medicinal plants, volume 2 (fruits). Springer, Dordrecht, pp 798–803CrossRefGoogle Scholar
  21. Macías C, Villaescusa R, del Valle L, Boffil V, Cordero G, Hernández A, Hernández P, Ballester JM (2003) Endothelial adhesion molecules ICAM-1, VCAM-1 and E-selectin in patients with acute coronary syndrome. Rev Esp Cardiol 56:137–144CrossRefGoogle Scholar
  22. Mahmood T, Yang PC (2012) Western blot: technique, theory, and troubleshooting. N Am J Med Sci 4:429–434CrossRefGoogle Scholar
  23. Miguel-Carrasco JL, Zambrano S, Blanca AJ, Mate A, Vázquez CM (2010) Captopril reduces cardiac inflammatory markers in spontaneously hypertensive rats by inactivation of NF-kB. J Inflamm 7:1–9CrossRefGoogle Scholar
  24. Miranda KM, Espey MG, Wink DA (2001) Rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5:62–71CrossRefGoogle Scholar
  25. Modlich U, Pugh CW, Bicknell R (2000) Increasing endothelial cell specific expression by the use of heterologous hypoxic and cytokine-inducible enhancers. Gene Ther 7:896–902CrossRefGoogle Scholar
  26. Mustafa NH, Ugusman A, Jalil J, Kamisah Y (2018) Anti-inflammatory properties of Parkia speciosa empty pod extract in human umbilical vein endothelial cells. J Appl Pharm Sci 8:152–158Google Scholar
  27. Rozaq P, Sofriani N (2009) Organic pesticide from urine and spices modification. Asian J Food Agro Ind Special Issue:S105–S111Google Scholar
  28. Sabio G, Davis RJ (2014) TNF and MAP kinase signaling pathways. Sem Immunol 26:237–245CrossRefGoogle Scholar
  29. Sambrook J, Fritsch EF, Maniatis T (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Inc., New YorkGoogle Scholar
  30. Samuel A, Kalusalingam A, Chellappan D, Gopinath R, Radhamani S, Husain H, Muruganandham V, Promwichit P (2010) Ethnomedical survey of plants used by the Orang Asli in Kampung Bawong, Perak, West Malaysia. J Ethnobiol Ethnomed 6:5CrossRefGoogle Scholar
  31. Siti HN, Kamisah Y, Kamsiah J (2015) The role of oxidative stress, antioxidants and vascular inflammation in cardiovascular disease (a review). Vascul Pharmacol 71:40–56CrossRefGoogle Scholar
  32. Sorriento D, Santulli G, Fusco A, Anastasio A, Trimarco B, Iaccarino G (2010) Intracardiac injection of AdGRK5-NT reduces left ventricular hypertrophy by inhibiting NF-κB-dependent hypertrophic gene expression. Hypertension 56:696–704CrossRefGoogle Scholar
  33. Strijdom H, Chamane N, Lochner A (2009) Nitric oxide in the cardiovascular system: a simple molecule with complex actions. Cardiovasc J Afr 20:303–310PubMedPubMedCentralGoogle Scholar
  34. Vaziri ND (2008) Causal link between oxidative stress, inflammation, and hypertension. Iran J Kidney Dis 2:1–10PubMedGoogle Scholar
  35. Verma IM (2004) Nuclear factor (NF)-κB proteins: therapeutic targets. Ann Rheum Dis 63:57–61CrossRefGoogle Scholar
  36. Xia F, Wang C, Jin Y, Liu Q, Meng Q, Liu K, Sun H (2014) Luteolin protects HUVECs from TNF-α-induced oxidative stress and inflammation via its effects on the Nox4/ROS-NF-κB and MAPK pathways. J Atheroscler Thromb 21:768–783CrossRefGoogle Scholar
  37. Yang W, Yu M, Fu J, Bao W, Wang D, Hao L, Yao P, Nüssler AK, Yan H, Liu L (2014) Deoxynivalenol induced oxidative stress and genotoxicity in human peripheral blood lymphocytes. Food Chem Toxicol 64:383–396CrossRefGoogle Scholar
  38. Yullia T (2008) Prakata. In: Variasi Masakan Petai & Jengkol, Tim Dapur DeMedia, DeMedia Pustaka, 1st edn. Jakarta Selatan, Indonesia, p 2Google Scholar
  39. Zhou-Stache J, Buettner R, Artmann G, Mittermayer C, Bosserhoff AK (2002) Inhibition of TNF-alpha induced cell death in human umbilical vein endothelial cells and Jurkat cells by protocatechuic acid. Med Biol Eng Comput 40:698–703CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Pharmacology, Faculty of Medicine, UKMMCUniversiti Kebangsaan MalaysiaCheras, Kuala LumpurMalaysia
  2. 2.Drug and Herbal Research Centre, Faculty of PharmacyUniversiti Kebangsaan MalaysiaKuala LumpurMalaysia
  3. 3.Department of Biochemistry, Faculty of Medicine, UKMMCUniversiti Kebangsaan MalaysiaCheras, Kuala LumpurMalaysia

Personalised recommendations