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Cytotechnology

, Volume 70, Issue 5, pp 1469–1477 | Cite as

Treatment of donor cells with recombinant KDM4D protein improves preimplantation development of cloned ovine embryos

  • Yumei Zhang
  • Qianqian Wang
  • Kailing Liu
  • Enen Gao
  • Hong Guan
  • Jian HouEmail author
Short Communication

Abstract

Incomplete epigenetic reprogramming is one of the major factors affecting the development of embryos cloned by somatic cell nuclear transfer (SCNT). Histone 3 lysine 9 (H3K9) trimethylation has been identified as a key barrier to efficient reprogramming by SCNT. The aim of this study was to explore a method of downregulating H3K9me3 levels in donor cells by using histone lysine demethylase (KDM) protein. When sheep fetal fibroblast cells were treated with recombinant human KDM4D protein (rhKDM4D), the levels of H3K9 trimethylation and dimethylation were both significantly decreased. After SCNT, rhKDM4D-treated donor cells supported significantly higher percentage of cloned embryos developing into blastocysts as compared to non-treated control cells. Moreover, the blastocyst quality was also improved by rhKDM4D treatment of donor cells, as assessed by the total cell number in blastocysts and the expression of developmental genes including SOX2, NANOG and CDX2. These results indicate that treatment of donor cells with recombinant KDM4D protein can downregulate the levels of H3K9 trimethylation and dimethylation and improve the developmental competence of SCNT embryos. This strategy may be convenient to be used in KDM4-assisted SCNT procedure for improving the efficiency of cloning.

Keywords

H3K9 methylation KDM4D Sheep Somatic cell nuclear transfer 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31172208) and China Agriculture Research System (Grant No. CARS-39-04).

Compliance with ethical standards

Conflict of interest

The authors declare no financial or commercial conflict of interest.

Supplementary material

10616_2018_224_MOESM1_ESM.pdf (293 kb)
Supplementary material 1 (PDF 292 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Agrobiotechnology, College of Biological ScienceChina Agricultural UniversityBeijingChina

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