Antidiabetic effects of novel cell culture established from grapevine, Vitis vinifera cv. Koshu
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Vitis vinifera cv. Koshu is an indigenous cultivar in Japan and has several characteristics that distinguish it from European V. vinifera. In Japan, Koshu is the most popular cultivar for wine making. We report herein a cell culture established from Koshu for use as a system for the production of resveratrol and its derivatives. Grape cell culture YU-1 was developed from the apex tissues of Koshu. YU-1 growth was favorably compared with BY-2 growth, a standard cell line in plant cell biology. Stilbene production and stilbene synthesis gene expression in YU-1 were upregulated by UV-C irradiation. YU-1 irradiated with UV-C decreased hemolymph sugar levels in model animals. Taken together, this study suggests that YU-1 may be used as a source of valuable medicinal components in plant cell bioreactor systems.
KeywordsAntidiabetic Hypoglycemic effect Koshu Resveratrol Vitis vinifera
We thank Dr. Hironori Kobayashi and Dr. Ryoji Takata (Kirin Company, Ltd.) for valuable discussion.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- Borie B, Jeandet P, Parize A, Bessis R, Adrian M (2004) Resveratrol and stilbene synthase mRNA production in grapevine levels treated with biotic and abiotic phytoalexin elicitors. Am J Enol Vitic 55:60–64Google Scholar
- Fujita K, Shimazaki M, Furiya T, Takayanagi T, Suzuki S (2009) Genetic variation among Koshu (Vitis vinifera L.) accessions generated by retrotransposon insertion into genome. Am J Enol Vitic 60:490–496Google Scholar
- Keskin N, Kunter B (2008) Production of trans-resveratrol in ‘Cabernet Sauvignon’ (Vitis vinifera L.) callus culture in response to ultraviolet-C irradiation. Vitis 47:193–196Google Scholar
- Yanai H (2011) Statcel, available: the useful add-in software 2 forms on Excel, 3rd edn. OMC, Tokyo, pp 172–175Google Scholar