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Cytotechnology

, Volume 70, Issue 3, pp 993–999 | Cite as

Antidiabetic effects of novel cell culture established from grapevine, Vitis vinifera cv. Koshu

  • Keiko Fujita
  • Yoshinao Aoki
  • Shunji SuzukiEmail author
Short Communication
  • 124 Downloads

Abstract

Vitis vinifera cv. Koshu is an indigenous cultivar in Japan and has several characteristics that distinguish it from European V. vinifera. In Japan, Koshu is the most popular cultivar for wine making. We report herein a cell culture established from Koshu for use as a system for the production of resveratrol and its derivatives. Grape cell culture YU-1 was developed from the apex tissues of Koshu. YU-1 growth was favorably compared with BY-2 growth, a standard cell line in plant cell biology. Stilbene production and stilbene synthesis gene expression in YU-1 were upregulated by UV-C irradiation. YU-1 irradiated with UV-C decreased hemolymph sugar levels in model animals. Taken together, this study suggests that YU-1 may be used as a source of valuable medicinal components in plant cell bioreactor systems.

Keywords

Antidiabetic Hypoglycemic effect Koshu Resveratrol Vitis vinifera 

Notes

Acknowledgements

We thank Dr. Hironori Kobayashi and Dr. Ryoji Takata (Kirin Company, Ltd.) for valuable discussion.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

References

  1. Belchí-Navarro S, Almagro L, Lijavetzky D, Bru R, Pedreño MA (2012) Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and methyljasmonate. Plant Cell Rep 31:81–89CrossRefPubMedGoogle Scholar
  2. Borie B, Jeandet P, Parize A, Bessis R, Adrian M (2004) Resveratrol and stilbene synthase mRNA production in grapevine levels treated with biotic and abiotic phytoalexin elicitors. Am J Enol Vitic 55:60–64Google Scholar
  3. Chung JH, Manganiello V, Dyck JRB (2012) Resveratrol as a calorie restriction mimetic: therapeutic implications. Trends Cell Biol 22:546–554CrossRefPubMedPubMedCentralGoogle Scholar
  4. Decendit A, Mérillon JM (1996) Condensed tannin and anthocyanin production in Vitis vinifera cell suspension cultures. Plant Cell Rep 15:762–765CrossRefPubMedGoogle Scholar
  5. Decendit A, Ramawat KG, Waffo P, Deffieux G, Badoc A, Mérillon JM (1996) Anthocyanins, catechins, condensed tannins and piceid production in Vitis vinifera cell bioreactor cultures. Biotechnol Lett 18:659–662CrossRefGoogle Scholar
  6. Delaunois B, Cordelier S, Conreux A, Clément C, Jeandet P (2009) Molecular engineering of resveratrol in plants. Plant Biotechnol J 7:2–12CrossRefPubMedGoogle Scholar
  7. Fernández-Mar MI, Mateos R, García-Parrilla MC, Puertas B, Cantos-Villar E (2012) Bioactive compounds in wine: resveratrol, hydroxytyrosol and melatonin: a review. Food Chem 130:797–813CrossRefGoogle Scholar
  8. Ferri M, Dipalo SCF, Bagni N, Tassoni A (2011) Chitosan elicits mono-glucosylated stilbene production and release in fed-batch bioreactor cultures of grape cells. Food Chem 124:1473–1479CrossRefGoogle Scholar
  9. Fujita K, Shimazaki M, Furiya T, Takayanagi T, Suzuki S (2009) Genetic variation among Koshu (Vitis vinifera L.) accessions generated by retrotransposon insertion into genome. Am J Enol Vitic 60:490–496Google Scholar
  10. Gagné S, Cluzet S, Mérillon JM, Gény L (2011) ABA initiates anthocyanin production in grape cell cultures. J Plant Growth Regul 30:1–10CrossRefGoogle Scholar
  11. Goto-Yamamoto N, Sawler J, Myles S (2015) Genetic analysis of east Asian grape cultivars suggests hybridization with wild Vitis. PLoS ONE 10:e0140841CrossRefPubMedPubMedCentralGoogle Scholar
  12. Keskin N, Kunter B (2008) Production of trans-resveratrol in ‘Cabernet Sauvignon’ (Vitis vinifera L.) callus culture in response to ultraviolet-C irradiation. Vitis 47:193–196Google Scholar
  13. Kobayashi H, Suzuki Y, Ajimura K, Konno T, Suzuki S, Saito H (2011) Characterization of phenolic compounds biosynthesized in pink-colored skin of Japanese indigenous Vitis vinifera cv. Koshu grape. Plant Biotechnol Rep 5:79–88CrossRefGoogle Scholar
  14. Larronde F, Krisa S, Decendit A, Chèze C, Deffieux G, Mérillon JM (1998) Regulation of polyphenol production in Vitis vinifera cell suspension cultures by sugars. Plant Cell Rep 17:946–950CrossRefGoogle Scholar
  15. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue culture. Physiol Plant 18:100–127CrossRefGoogle Scholar
  16. Matsumoto Y, Sumiya E, Sugita T, Sekimizu K (2011) An invertebrate hyperglycemic model for the identification of anti-diabetic drugs. PLoS ONE 6:e18292CrossRefPubMedPubMedCentralGoogle Scholar
  17. Nivelle L, Hubert J, Courot E, Jeandet P, Aziz A, Nuzillard JM, Renault JH, Clément C, Martiny L, Delmas D, Tarpin M (2017) Anti-cancer activity of resveratrol and derivatives produced by grapevine cell suspensions in a 14 L stirred bioreactor. Molecules 22:474CrossRefGoogle Scholar
  18. Pandey KB, Rizvi SI (2014) Role of red grape polyphenols as antidiabetic agents. Integr Med Res 3:119–125CrossRefPubMedPubMedCentralGoogle Scholar
  19. Santamaria AR, Mulinacci N, Valletta A, Innocenti M, Pasqua G (2011) Effects of elicitors on the production of resveratrol and viniferins in cell cultures of Vitis vinifera L. cv Italia. J Agric Food Chem 59:9094–9101CrossRefPubMedGoogle Scholar
  20. Shimazaki M, Fujita K, Kobayashi H, Suzuki S (2011) Pink-colored grape berry is the result of short insertion in intron of color regulatory gene. PLoS ONE 6:e21308CrossRefPubMedPubMedCentralGoogle Scholar
  21. Szkudelski T, Szkudelska K (2011) Anti-diabetic effects of resveratrol. Ann N Y Acad Sci 1215:34–39CrossRefPubMedGoogle Scholar
  22. Takayanagi T, Okuda T, Mine Y, Yokotsuka K (2004) Induction of resveratrol biosynthesis in skins of three grape cultivars by ultraviolet irradiation. J Jpn Soc Hortic Sci 73:193–199CrossRefGoogle Scholar
  23. Yanai H (2011) Statcel, available: the useful add-in software 2 forms on Excel, 3rd edn. OMC, Tokyo, pp 172–175Google Scholar
  24. Zamboni A, Gatto P, Cestaro A, Pilati S, Viola R, Mattivi F, Moser C, Velasco R (2009) Grapevine cell early activation of specific responses to DIMEB, a resveratrol elicitor. BMC Genom 10:363CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Life and Environmental SciencesPrefectural University of HiroshimaHiroshimaJapan
  2. 2.Laboratory of Fruit Genetic Engineering, The Institute of Enology and ViticultureUniversity of YamanashiKofuJapan

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