Friedelane, isolated from Pouzolzia indica Gaud. exhibits toxic effect against melanoma

  • Debasish Maiti
  • Ashish Kumar Singha
  • Chaitali Sarkar
  • Soham Sarkar
  • Indrajit Sil Sarma
  • Kuntal Manna
  • Biswanath Dinda
Original Article

Abstract

Melanoma is a predominant cause of skin cancer-related deaths. It was reported that, the methanolic extract of Pouzolzia Indica (P. indica) on chromatography gave five compounds (1-hentriacontanyl palmitate, myricyl alcohol, 6,7-dimethoxycoumarin, trichadonic acid and friedelane), which inhibited the acute promyelocytic leukemia cell lines, NB4, and HT93A. Friedelane was extracted as active compound from methanolic extract of P. indica. In this study, friedelane was tested on murine metastatic B16F10 and B16BL6 melanoma cell lines. To achieve the target, the cell viability using trypan blue exclusion, acridine orange/EtBr staining and cell cytotoxicity were tested using MTT assay. Caspase-3, caspase-9, Cyt-c, BAD and Bax protein were assayed to evidence the apoptosis induction. The compound friedelane shows potent cytotoxic effect against metastatic melanoma mouse cell lines in 10 µg/ml concentration.

Keywords

Pauzolzia indica Gaud. Friedelane (Fr) Melanoma Cell viability Cytotoxicity Apoptosis 

Notes

Acknowledgements

We are thankful to DBT for funding and State Biotech Hub, Tripura University for providing the instrumental facilities. We are also thankful to ICMR for providing fellowship to AKS to complete this work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest for this study.

Supplementary material

10616_2017_174_MOESM1_ESM.doc (423 kb)
Supplementary material 1 (DOC 423 kb)
10616_2017_174_MOESM2_ESM.doc (461 kb)
Supplementary material 2 (DOC 461 kb)
10616_2017_174_MOESM3_ESM.doc (462 kb)
Supplementary material 3 (DOC 462 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Debasish Maiti
    • 1
  • Ashish Kumar Singha
    • 1
  • Chaitali Sarkar
    • 1
  • Soham Sarkar
    • 2
  • Indrajit Sil Sarma
    • 3
  • Kuntal Manna
    • 4
  • Biswanath Dinda
    • 5
  1. 1.Immunology Microbiology Lab, Department of Human PhysiologyTripura UniversityAgartalaIndia
  2. 2.Wilson’s SchoolWallingtonUK
  3. 3.Tripura State Forensic Science LaboratoryNarasingarh, AgartalaIndia
  4. 4.Department of PharmacyTripura UniversityAgartalaIndia
  5. 5.Department of ChemistryTripura UniversityAgartalaIndia

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