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Cytotechnology

, Volume 70, Issue 3, pp 1009–1021 | Cite as

Biological characterization and pluripotent identification of ovine amniotic fluid stem cells

  • Wenhua Pei
  • Tengfei Lu
  • Kunfu Wang
  • Meng Ji
  • Shuang Zhang
  • Fenghao Chen
  • Lu Li
  • Xiangchen Li
  • Weijun Guan
Original Article

Abstract

Mesenchymal stem cells derived from amniotic fluid have become one of the most potential stem cell source for cell-based therapy for the reason they can be harvested at low cost and without ethical problems. Here, we obtained amniotic fluid stem cells (AFSCs) from ovine amniotic fluid and studied the expansion capacity, cell markers expression, karyotype, and multilineage differentiation ability. In our work, AFSCs were subcultured to passage 62. The cell markers, CD29, CD44, CD73 and OCT4 which analyzed by RT-PCR were positive; CD44, CD73, CD90, CD105, NANOG, OCT4 analyzed by immunofluorescence and flow cytometry were also positive. The growth curves of different passages were all typically sigmoidal. The different passages cells took on a normal karyotype. In addition, AFSCs were successfully induced to differentiate into adipocytes, osteoblasts and chondrocytes. The results suggested that the AFSCs isolated from ovine maintained normal biological characteristics and their multilineage differentiation potential provides many potential applications in cell-based therapies and tissue engineering.

Keywords

Ovine Amniotic fluid Stem cell Differentiation 

Notes

Acknowledgements

This research was supported by the Project supported by the National Natural Science Foundation of China (Grant Nos. 31472099, 31672404).

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interests regarding the publication of this paper.

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Copyright information

© Springer Science+Business Media B.V. 2018

Authors and Affiliations

  • Wenhua Pei
    • 1
  • Tengfei Lu
    • 1
  • Kunfu Wang
    • 2
  • Meng Ji
    • 1
  • Shuang Zhang
    • 3
  • Fenghao Chen
    • 4
  • Lu Li
    • 5
  • Xiangchen Li
    • 1
  • Weijun Guan
    • 1
  1. 1.Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijingChina
  2. 2.College of Wildlife ResourcesNortheast Forestry UniversityHarbinChina
  3. 3.Research Center for Sports Scientific ExperimentHarbin Sport UniversityHarbinPeople’s Republic of China
  4. 4.College of Human Movement ScienceHarbin Sport UniversityHarbinChina
  5. 5.College of Animal Science and TechnologyAgricultural University of HebeiBaodingChina

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