Cytotechnology

, Volume 68, Issue 4, pp 1447–1461 | Cite as

A comparative study on expression profile of developmentally important genes during pre-implantation stages in buffalo hand-made cloned embryos derived from adult fibroblasts and amniotic fluid derived stem cells

  • Sadeesh EM
  • Fozia Shah
  • Meena Kataria
  • P. S. Yadav
Original Article
First Online:
Received:
Accepted:

Abstract

Abnormal gene expression in somatic cell nuclear transfer embryos due to aberrant epigenetic modifications of the donor nucleus may account for much of the observed diminished viability and developmental abnormalities. The present study compared the developmentally important gene expression pattern at 4-cell, 8- to 16-cell, morula, and blastocyst stages of buffalo nuclear transfer (NT) embryos from adult fibroblasts (AFs) and amniotic fluid stem cells (AFSCs). In vitro fertilized embryos were used as control embryos. Alterations in the expression pattern of genes implicated in transcription and pluripotency (OCT4, STAT3, NANOG), DNA methylation (DNMT1, DNMT3A), histone deacetylation (HDAC2), growth factor signaling, and imprinting (IGF2, IGF2R), apoptosis (BAX, BCL2), oxidative stress (MnSOD), metabolism (GLUT1) regulation were observed in cloned embryos. The expression of transcripts in AFSC-NT embryos more closely followed that of the in vitro fertilized embryos compared with AF-NT embryos. It is concluded that AFSCs with a relatively undifferentiated genome may serve as suitable donors which could be reprogrammed more efficiently to reactivate expression of early embryonic genes in buffalo NT.

Keywords

Buffalo Adult fibroblasts Amniotic fluid stem cells Hand-made cloning Gene expression 
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Notes

Acknowledgments

The authors would like to thank Dr. BS Punia, ICAR-Central Institute for Research on Buffaloes, for reviewing the manuscript. Funding support from Indian Council of Agricultural Research (ICAR), New Delhi is gratefully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Sadeesh EM
    • 1
  • Fozia Shah
    • 2
  • Meena Kataria
    • 3
  • P. S. Yadav
    • 1
  1. 1.Division of Animal Physiology and ReproductionICAR-Central Institute for Research on BuffaloesHisarIndia
  2. 2.Department of Veterinary Physiology and BiochemistryLala Lajpat Rai University of Veterinary and Animal SciencesHisarIndia
  3. 3.Division of BiochemistryICAR-Indian Veterinary Research InstituteBareillyIndia

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