, Volume 68, Issue 4, pp 1425–1433 | Cite as

Astrocyte/neuron ratio and its importance on glutamate toxicity: an in vitro voltammetric study

  • Ahmet HacimuftuogluEmail author
  • Abdulgani Tatar
  • Damla Cetin
  • Numan Taspinar
  • Fatih Saruhan
  • Ufuk Okkay
  • Hasan Turkez
  • Deniz Unal
  • Robert Louis StephensJr.
  • Halis Suleyman
Original Article


The purpose of this study was to clarify the relationship between neuron cells and astrocyte cells in regulating glutamate toxicity on the 10th and 20th day in vitro. A mixed primary culture system from newborn rats that contain cerebral cortex neurons cells was employed to investigate the glutamate toxicity. All cultures were incubated with various glutamate concentrations, then viability tests and histological analyses were performed. The activities of glutamate transporters were determined by using in vitro voltammetry technique. Viable cell number was decreased significantly on the 10th day at 10−7 M and at 10−6 M glutamate applications, however, viable cell number was not decreased at 20th day. Astrocyte number was increased nearly six times on the 20th day as compared to the 10th day. The peak point of glutamate reuptake capacity was about 2 × 10−4 M on the 10th day and 10−3 M on the 20th day. According to our results, we suggested that astrocyte age was important to maintain neuronal survival against glutamate toxicity. Thus, we revealed activation or a trigger point of glutamate transporters on astrocytes due to time since more glutamate was taken up by astrocytes when glutamate transporters on the astrocyte were triggered with high exogenous glutamate concentrations. In conclusion, the present investigation is the first voltammetric study on the reuptake parameters of glutamate in vitro.


Glutamate-uptake Neurotoxicity Astrocyte Neuron In vitro voltammetry 



This study was supported by grants from The Scientific and Technological Research Council of Turkey (TUBİTAK-Project No: 107S067) and Atatürk University BAP (Project No: 2005/160 and 2011/270-271)

Conflict of interest

The authors declared that there are no conflicts of interest.


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Ahmet Hacimuftuoglu
    • 1
    Email author
  • Abdulgani Tatar
    • 2
  • Damla Cetin
    • 3
  • Numan Taspinar
    • 1
  • Fatih Saruhan
    • 1
  • Ufuk Okkay
    • 1
  • Hasan Turkez
    • 4
    • 5
  • Deniz Unal
    • 6
  • Robert Louis StephensJr.
    • 7
  • Halis Suleyman
    • 8
  1. 1.Department of Medical Pharmacology, Faculty of MedicineAtatürk UniversityErzurumTurkey
  2. 2.Department of Medical Genetics, Faculty of MedicineAtaturk UniversityErzurumTurkey
  3. 3.Department of Medical Pharmacology, Faculty of MedicineKafkas UniversityKarsTurkey
  4. 4.Department of Molecular Biology and Genetics, Faculty of SciencesErzurum Technical UniversityErzurumTurkey
  5. 5.Department of Pharmaceutical SciencesUniversity of “G. D’Annunzio”ChietiItaly
  6. 6.Department of Histology, Faculty of MedicineAtaturk UniversityErzurumTurkey
  7. 7.Department of Physiology and Cell Biology, Medical CollegeThe Ohio State UniversityColumbusUSA
  8. 8.Department of Medical Pharmacology, Faculty of MedicineErzincan UniversityErzincanTurkey

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