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Cytotechnology

, Volume 68, Issue 4, pp 1147–1159 | Cite as

Transgene expression in Penaeus monodon cells: evaluation of recombinant baculoviral vectors with shrimp specific hybrid promoters

  • Jayesh Puthumana
  • Rosamma Philip
  • I. S. Bright Singh
Original Research

Abstract

It has been realized that shrimp cell immortalization may not be accomplished without in vitro transformation by expressing immortalizing gene in cells. In this process, efficiency of transgene expression is confined to the ability of vectors to transmit gene of interests to the genome. Over the years, unavailability of such vectors has been hampering application of such a strategy in shrimp cells. We report the use of recombinant baculovirus mediated transduction using hybrid promoter system for transgene expression in lymphoid cells of Penaeus monodon. Two recombinant baculovirus vectors with shrimp viral promoters (WSSV-Ie1 and IHHNV-P2) were constructed (BacIe1-GFP and BacP2-GFP) and green fluorescent protein (GFP) used as the transgene. The GFP expression in cells under the control of hybrid promoters, PH-Ie1 or PH-P2, were analyzed and confirmed in shrimp cells. The results indicate that the recombinant baculovirus with shrimp specific viral promoters (hybrid) can be employed for delivery of foreign genes to shrimp cells for in vitro transformation.

Keywords

Transgene expression Penaeus monodon Shrimp specific vectors Hybrid promoter Baculoviral vectors 

Notes

Acknowledgments

This research was supported by the Department of Biotechnology, Government of India (BT/PR8050/AAQ/03/289/2006 and BT/PR5126/AAQ/3/591/2012). The first author thanks DBT for Fellowship.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Jayesh Puthumana
    • 1
  • Rosamma Philip
    • 2
  • I. S. Bright Singh
    • 1
  1. 1.National Centre for Aquatic Animal HealthCochin University of Science and TechnologyKochiIndia
  2. 2.Department of Marine Biology, Microbiology and BiochemistryCochin University of Science and TechnologyKochiIndia

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