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Cytotechnology

, Volume 68, Issue 4, pp 1115–1122 | Cite as

Components of chicken egg white extract smaller than 3 kDa in size promote 293T cell proliferation

  • Guang-ping RuanEmail author
  • Xiang Yao
  • Jin-xiang Wang
  • Ju-fen Liu
  • Fan Shu
  • Zi-an Li
  • Rong-qing Pang
  • Xing-hua PanEmail author
Original Research
  • 174 Downloads

Abstract

We previously found that chicken egg white extract could promote cell survival and proliferation. In the present study, we further separated this extract into its components to identify those primarily responsible for promoting cell proliferation. Components of differing molecular weight were separated from chicken egg white extract by ultrafiltration and 293T cell cultures were supplemented with various concentrations. The effects on cell proliferation were subsequently determined by a CellTiter 96 Aqueous One Solution Cell Proliferation Assay kit (Promega). We demonstrate that components from chicken egg white smaller than 3 kDa in size are able to function as active ingredients promoting cellular proliferation. This discovery may identify a new and convenient additive for cell culture media to promote cell growth and proliferation.

Keywords

Chicken egg white extract Active ingredients Cell proliferation Separation 

Notes

Acknowledgments

This work was supported by National Support Program (2014BAI01B01) and Yunnan Provincial Science and Technology Project (2013CA005). We thank American Journal Experts for assisting in the preparation of this manuscript.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Guang-ping Ruan
    • 1
    • 2
    • 3
    Email author
  • Xiang Yao
    • 1
    • 2
    • 3
  • Jin-xiang Wang
    • 1
    • 2
    • 3
  • Ju-fen Liu
    • 1
    • 2
    • 3
  • Fan Shu
    • 1
    • 2
    • 3
  • Zi-an Li
    • 1
    • 2
    • 3
  • Rong-qing Pang
    • 1
    • 2
    • 3
  • Xing-hua Pan
    • 1
    • 2
    • 3
    Email author
  1. 1.The Cell Biological Therapy CenterKunming General Hospital of Chengdu Military CommandKunmingChina
  2. 2.Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province)KunmingChina
  3. 3.Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan ProvinceKunmingChina

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