Oridonin suppress cell migration via regulation of nonmuscle myosin IIA
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Oridonin, which is isolated from Chinese herb Rabdosia rubescens (Hemsl.) Hara, has been implicated in regulation of tumor cell migration and invasion. In this study, treatment with oridonin enhanced the phosphorylation of myosin regulatory light chain (T18/S19) that regulates the ATPase activity of myosin IIA. Meanwhile, stress fibers were significantly more prominent after oridonin incubation, which impaired cell migration in transwell migration assays. All of these effects may be caused by the decreased interaction between myosin IIA and myosin phosphatase complex, but not kinases. Our data provide clear evidence of this novel pharmacological function for oridonin in treating cancer cell migration.
KeywordsOridonin Migration Myosin IIA Regulatory light chain Phosphatase complex
Myosin light chain kinase
Myosin phosphatase-targeting subunit 1
- NM II
Nonmuscle myosin II
Nonmuscle myosin heavy chain
Catalytic subunit of protein phosphatase type 1δ
Regulatory light chain
Rho-associated protein kinase
This work was supported by the National Natural Science Foundation of China (NSFC No. 81172961).
Conflict of interest
The authors declare that there are no conflicts of interest.
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