Cytotechnology

, Volume 68, Issue 3, pp 389–397 | Cite as

Oridonin suppress cell migration via regulation of nonmuscle myosin IIA

Original Research

Abstract

Oridonin, which is isolated from Chinese herb Rabdosia rubescens (Hemsl.) Hara, has been implicated in regulation of tumor cell migration and invasion. In this study, treatment with oridonin enhanced the phosphorylation of myosin regulatory light chain (T18/S19) that regulates the ATPase activity of myosin IIA. Meanwhile, stress fibers were significantly more prominent after oridonin incubation, which impaired cell migration in transwell migration assays. All of these effects may be caused by the decreased interaction between myosin IIA and myosin phosphatase complex, but not kinases. Our data provide clear evidence of this novel pharmacological function for oridonin in treating cancer cell migration.

Keywords

Oridonin Migration Myosin IIA Regulatory light chain Phosphatase complex 

Abbreviations

MLCK

Myosin light chain kinase

MYPT1

Myosin phosphatase-targeting subunit 1

NM II

Nonmuscle myosin II

NMHC

Nonmuscle myosin heavy chain

PP1cδ

Catalytic subunit of protein phosphatase type 1δ

RLC

Regulatory light chain

ROCK

Rho-associated protein kinase

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (NSFC No. 81172961).

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.School of Pharmaceutical SciencesZhengzhou UniversityZhengzhouPeople’s Republic of China

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