Cytotechnology

, Volume 68, Issue 3, pp 371–379 | Cite as

The effects of different molecular weight chondroitin-4-sulfates in chondrocyte pellet culture

  • Shu-Rui Yang
  • Sydney Peng
  • Chao-Yin Ko
  • I-Ming Chu
Original Research
First Online:
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Accepted:
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Abstract

For this study, we cultured chondrocyte pellets in Dulbecco’s modified Eagle’s medium plus a 2 % fetal bovine serum medium, and treated them with 2- to 8-mer oligosaccharides of chondroitin sulfate A to examine the effects of these oligosaccharides on the differentiation and protection of chondrocytes. We found low-molecular-weight CSAs to increase the ratio of the gene expression levels of collagen II/collagen I of chondrocytes from the first day up to 14 days after culture compared with those under a CSA-free medium. Moreover, low-molecular-weight CSAs inhibited the expression of matrix metalloproteinases and peptidases, and stimulated an endogenous tissue inhibitor of metalloproteinases. The dp-8 (8-mer) CSA yielded the most effective response among promoting collagen type II protein secretions compared with other groups.

Keywords

Chondroitin-4-sulfate Chondrocyte Collagen Metalloproteinase Gene 

Abbreviations

dp-2

2-mers Oligosaccharides

dp-4

4-mers Oligosaccharides

dp-8

8-mers Oligosaccharides

ADAMTS

A disintegrin and metalloproteinase with a thrombospondin type 1 motif

AC

Articular cartilage

COL II

Type II collagen

COL I

Type I collagen

CS

Chondroitin sulfate

CSA

Chondroitin sulfate A

CSC

Chondroitin-6-sulfate

DS

Dermatan sulfate

ECM

Extracellular matrix

GAGs

Glycosaminoglycan

MMPS

Metalloproteinase

OA

Osteoarthritis

PGs

Proteoglycans

TIMP3

Tissue inhibitor of matrix metalloproteinase

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Notes

Acknowledgments

This research was partially supported by funding from the Ministry of Economic Affairs, under Grant 91-EC-17-A-17-S1-0009.

Supplementary material

10616_2014_9788_MOESM1_ESM.pdf (94 kb)
Table 1. Gel permeation chromatography (GPC) of digest GAGs. (PDF 93 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Shu-Rui Yang
    • 1
  • Sydney Peng
    • 1
  • Chao-Yin Ko
    • 1
  • I-Ming Chu
    • 1
  1. 1.Department of Chemical EngineeringNational Tsing Hua UniversityHsinchuTaiwan, ROC

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