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Cytotechnology

, Volume 68, Issue 1, pp 143–155 | Cite as

Pyocyanin induced in vitro oxidative damage and its toxicity level in human, fish and insect cell lines for its selective biological applications

  • P. Priyaja
  • P. Jayesh
  • Rosamma Philip
  • I. S. Bright Singh
Original Research

Abstract

Pyocyanin is a redox active phenazine pigment produced by Pseudomonas aeruginosa, with broad antibiotic activity having pharmacological, aquaculture, agriculture and industrial applications. In the present work cytotoxicity induced by pyocyanin is demonstrated in a human embryonic lung epithelial cell line (L-132), a rainbow trout gonad cell line (RTG-2) and a Spodoptera frugiperda pupal ovarian cell line (Sf9). For toxicity evaluation, cellular morphology, mitochondrial function (XTT), membrane leakage of lactate dehydrogenase, neutral red uptake, affinity of electrostatic binding of protein with sulforhodamine B dyes, glucose metabolism, and reactive oxygen species, were assessed. Results showed that higher pyocyanin concentration is required for eliciting cytotoxicity in L-132, RTG-2 and Sf9. The microscopic studies demonstrated that the cell lines exposed to pyocyanin at higher concentrations alone showed morphological changes such as clumping and necrosis. Among the three cell lines L-132 showed the highest response to pyocyanin than the others. In short, pyocyanin application at concentrations ranging from 5 to 10 mg l−1 were not having any pathological effect in eukaryotic systems and can be used as drug of choice in aquaculture against vibrios in lieu of conventional antibiotics and as biocontrol agent against fungal and bacterial pathogens in agriculture. This is besides its industrial and pharmacological applications.

Keywords

Pyocyanin Toxicity IC50 Vibriosis Antibacterial Antifungal 

Notes

Acknowledgments

This work was funded by Department of Biotechnology, Government of India under the project, Program Support in Marine Biotechnology (Order No.: BT/PR4012/AAQ/03/204/2003). The first author thanks DBT for Fellowship.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • P. Priyaja
    • 1
  • P. Jayesh
    • 1
  • Rosamma Philip
    • 2
  • I. S. Bright Singh
    • 1
  1. 1.National Centre for Aquatic Animal HealthCochin University of Science and TechnologyKochiIndia
  2. 2.Department of Marine Biology, Microbiology and Biochemistry, School of Marine SciencesCochin University of Science and TechnologyKochiIndia

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