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Cytotechnology

, Volume 68, Issue 1, pp 135–142 | Cite as

Phenolic contents and antioxidant activity of ethanolic extract of Capparis spinosa

  • Riadh Ben Mansour
  • Imtinen Ben Haj Jilani
  • Mohammed Bouaziz
  • Bochra Gargouri
  • Nésrine Elloumi
  • Hamadi Attia
  • Zeineb Ghrabi-Gammar
  • Saloua Lassoued
Original Research

Abstract

Caper plant (Capparis spinosa) extracts have been associated with diverse biological activities including anti-oxidant properties. In this work, we characterized the hydro-ethanolic extract obtained from C. spinosa leaves [hydroethanolic extract of C. spinosa (HECS)] by analyzing the content in anti-oxidant compounds such as polyphenols, flavonoids and anthocyanins. Further, we evaluated HECS antioxidant activities in vitro using bleaching of 1,1-diphenyl-2-picrylhydrazyl radical and ABTS test as well as by pretreatment of HeLa cells exposed to Fe2+ or H2O2. Our findings indicate that HECS contains high amount of total phenolic compounds and high levels of flavonoids and anthocyanins. Furthermore, HECS exhibited antioxidant activity in both chemical and biological tests. Specially, pretreatment of HeLa cells with different concentrations of the extract conferred protection against lipid peroxidation and modulated activities of two antioxidant enzymes, SOD and catalase. These results revealed HECS antioxidant effects and suggest that C. spinosa leaves are a potential source of natural antioxidant molecules with possible applications in industry and medicine.

Keywords

Caper (Capparis spinosaPhenolic compounds Ethanol extract Antioxidant activity Lipid peroxidation (LPO) 

Abbreviations

ABTS

2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)

DM

Dry matter

DPPH

1,1-Diphenyl-2-picrylhydrazyl

FCS

Foetal calf serum

GAE

Gallic acid equivalent

HECS

Hydroethanolic extract of Capparis spinosa

MDA

Malondialdehyde

CS

Capparis spinosa

PBS

Phosphate buffer saline

ROS

Reactive oxygen species

SOD

Superoxide dismutase

TBA

Thiobarbuturic acid

TBARs

Thiobarbuturic acid reactive species

TEAC

Trolox equivalent antioxidant capacity

TEP

1,1,3,3-Tetraethoxypropane

Notes

Acknowledgments

The present investigation was supported financially by the ministry of high education and scientific research—TUNISIA.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Riadh Ben Mansour
    • 1
  • Imtinen Ben Haj Jilani
    • 2
  • Mohammed Bouaziz
    • 3
  • Bochra Gargouri
    • 1
  • Nésrine Elloumi
    • 1
  • Hamadi Attia
    • 1
  • Zeineb Ghrabi-Gammar
    • 2
  • Saloua Lassoued
    • 1
  1. 1.Laboratoire d’analyse, valorisation et sécurité des aliments, groupe de recherche «Biotechnologie et pathologies», Institut Supérieur de Biotechnologie de SfaxUniversité de SfaxSfaxTunisia
  2. 2.Unité de Recherche Biogéographie, Climatologie Appliquée et Dynamique Erosive, Faculté des Lettres, Institut National Agronomique de Tunisiedes Arts et des Humanités de ManoubaSfaxTunisia
  3. 3.Laboratoire des Bioprocédés Environnementaux, Centre de Biotechnologie de SfaxUniversité de SfaxSfaxTunisia

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