, Volume 67, Issue 6, pp 939–946 | Cite as

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

  • Basak TogarEmail author
  • Hasan Turkez
  • Abdulgani Tatar
  • Ahmet Hacimuftuoglu
  • Fatime Geyikoglu
Brief Report


The main objective of this study is to investigate the cytotoxic, genotoxic and antioxidant properties of zingiberene (ZBN) in an in vitro rat brain cell culture study. The cytotoxic effect was determined against the rat neuron and N2a neuroblastoma (N2a-NB) cell lines using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, while the antioxidant activity was assessed using the total antioxidant capacity (TAC) and total oxidative stress (TOS) assays. The effects on DNA damage were also evaluated in this study by the single cell gel electrophoresis assay. The results indicated that ZBN has an anti-proliferative activity suppressing the proliferation of N2a-NB cells at concentrations over 50 mg L−1 and neuron cells at concentrations over 150 mg L−1. In addition, ZBN treatments at higher doses (≤50 mg L−1) led to increases of TOS levels in N2a-NB cell cultures. However 25 mg L−1 of ZBN treatment caused increases of TAC levels in cultured neuron and N2a-NB cell cultures while ZBN at doses of 10–400 mg L−1 did not increase the number of total damage score in both cell lines. This study clearly indicates that ZBN has a significant potential to be used as a natural anticancer agent in cultured N2a-NBs.


Zingiberene N2a neuroblastoma Total oxidative stress Total antioxidant capacity MTT assay Single cell gel electrophoresis 



This work was supported by the Scientific & Technological Research Council of Turkey (TÜBİTAK, Project Number: 210T142).


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Basak Togar
    • 1
    Email author
  • Hasan Turkez
    • 2
  • Abdulgani Tatar
    • 3
  • Ahmet Hacimuftuoglu
    • 4
  • Fatime Geyikoglu
    • 1
  1. 1.Department of Biology, Faculty of ScienceAtatürk UniversityErzurumTurkey
  2. 2.Department of Molecular Biology and Genetics, Faculty of SciencesErzurum Technical UniversityErzurumTurkey
  3. 3.Department of Medical Genetics, Faculty of MedicalAtatürk UniversityErzurumTurkey
  4. 4.Department of Medical Pharmacology, Faculty of MedicalAtatürk UniversityErzurumTurkey

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