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Cytotechnology

, Volume 67, Issue 3, pp 559–571 | Cite as

Multifactorial interaction of growth factors on Penaeus monodon lymphoid cells and the impact of IGFs in DNA synthesis and metabolic activity in vitro

  • P. Jayesh
  • Rosamma Philip
  • I. S. Bright Singh
Method in Cell Science

Abstract

Development of continuous cell lines from shrimp is essential to investigate viral pathogens. Unfortunately, there is no valid cell line developed from crustaceans in general and shrimps in particular to address this issue. Lack of information on the requirements of cells in vitro limits the success of developing a cell line, where the microenvironment of a cell culture, provided by the growth medium, is of prime importance. Screening and optimization of growth medium components based on statistical experimental designs have been widely used for improving the efficacy of cell culture media. Accordingly, we applied Plackett–Burman design and response surface methodology to study multifactorial interactions between the growth factors in shrimp cell culture medium and to identify the most important ones for growth of lymphoid cell culture from Penaeus monodon. The statistical screening and optimization indicated that insulin like growth factor-I (IGF-I) and insulin like growth factor-II (IGF-II) at concentrations of 100 and 150 ng ml−1, respectively, could significantly influence the metabolic activity and DNA synthesis of the lymphoid cells. An increase of 53 % metabolic activity and 24.8 % DNA synthesis could be obtained, which suggested that IGF-I and IGF-II had critical roles in metabolic activity and DNA synthesis of shrimp lymphoid cells.

Keywords

Growth factor optimization Response surface methodology Plackett–Burman Central composite design Penaeus monodon Lymphoid cell culture Shrimp cell culture medium 

Notes

Acknowledgments

This research was supported by Department of Biotechnology, Government of India (BT/PR8050/AAQ/03/289/2006). The first author thanks DBT for Fellowship.

Supplementary material

10616_2014_9697_MOESM1_ESM.doc (352 kb)
Supplementary material 1 (DOC 352 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • P. Jayesh
    • 1
  • Rosamma Philip
    • 2
  • I. S. Bright Singh
    • 1
  1. 1.National Centre for Aquatic Animal HealthCochin University of Science and TechnologyKochiIndia
  2. 2.Department of Marine Biology, Microbiology and BiochemistryCochin University of Science and TechnologyKochiIndia

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