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Cytotechnology

, Volume 66, Issue 1, pp 159–168 | Cite as

Monitoring of the effects of transfection with baculovirus on Sf9 cell line and expression of human dipeptidyl peptidase IV

  • Özlem Üstün-AytekinEmail author
  • İsmet Deliloğlu Gürhan
  • Kayoko Ohura
  • Teruko Imai
  • Gaye Öngen
Original Research

Abstract

Human dipeptidylpeptidase IV (hDPPIV) is an enzyme that is in hydrolase class and has various roles in different parts of human body. Its deficiency may cause some disorders in the gastrointestinal, neurologic, endocrinological and immunological systems of humans. In the present study, hDPPIV enzyme was expressed on Spodoptera frugiperda (Sf9) cell lines as a host cell, and the expression of hDPPIV was obtained by a baculoviral expression system. The enzyme production, optimum multiplicity of infection, optimum transfection time, infected and uninfected cell size and cell behavior during transfection were also determined. For maximum hDPPIV (269 mU mL−1) enzyme, optimum multiplicity of infection (MOI) and time were 0.1 and 72 h, respectively. The size of infected cells increased significantly (P < 0.001) after 24 h post infection. The results indicated that Sf9 cell line was applicable to the large scale for hDPPIV expression by using optimized parameters (infection time and MOI) because of its high productivity (4.03 mU m L−1 h−1).

Keywords

Spodoptera frugiperda (Sf9) Dipeptidyl peptidase IV (DPPIV) Baculovirus Transfection 

Notes

Acknowledgments

This research was partly supported by grant from Japan Student Service Organization (JASSO).

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Özlem Üstün-Aytekin
    • 1
    • 2
    • 3
    Email author
  • İsmet Deliloğlu Gürhan
    • 2
  • Kayoko Ohura
    • 3
  • Teruko Imai
    • 3
  • Gaye Öngen
    • 2
  1. 1.Department of Food Engineering, Faculty of EngineeringPamukkale UniversityKinikli, DenizliTurkey
  2. 2.Bioengineering Department, Faculty of EngineeringEge UniversityBornova, IzmirTurkey
  3. 3.Graduate School of Pharmaceutical SciencesKumamoto UniversityKumamotoJapan

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