, Volume 66, Issue 1, pp 87–94 | Cite as

Effect of Ottoman Viper (Montivipera xanthina (Gray, 1849)) Venom on Various Cancer Cells and on Microorganisms

  • Husniye Tansel Yalcın
  • Mehmet Ozgün Ozen
  • Bayram Gocmen
  • Ayse NalbantsoyEmail author
Original Research


Cytotoxic and antimicrobial effects of Montivipera xanthina venom against LNCaP, MCF-7, HT-29, Saos-2, Hep3B, Vero cells and antimicrobial activity against selected bacterial and fungal species: Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, E. coli O157H7, Enterococcus faecalis 29212, Enterococcus faecium DSM 13590, Staphylococcus epidermidis ATCC 12228, S. typhimirium CCM 5445, Proteus vulgaris ATCC 6957 and Candida albicans ATCC 10239 were studied for evaluating the potential medical benefit of this snake venom. Cytotoxicity of venom was determined using MTT assay. Snake venom cytotoxicity was expressed as the venom dose that killed 50 % of the cells (IC50). The antimicrobial activity of venom was studied by minimal inhibitory concentration (MIC) and disc diffusion assay. MIC was determined using broth dilution method. The estimated IC50 values of venom varied from 3.8 to 12.7 or from 1.9 to 7.2 μg/ml after treatment with crude venom for 24 or 48 h for LNCaP, MCF-7, HT-29 and Saos-2 cells. There was no observable cytotoxic effect on Hep3B and Vero cells. Venom exhibited the most potent activity against C. albicans (MIC, 7.8 μg/ml and minimal fungicidal concentration, 62.5 μg/ml) and S. aureus (MIC, 31.25 μg/ml). This study is the first report showing the potential of M. xanthina venom as an alternative therapeutic approach due to its cytotoxic and antimicrobial effects.


Snake venom Cytotoxicity Antimicrobial activity Viper Montivipera xanthina 


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Husniye Tansel Yalcın
    • 1
  • Mehmet Ozgün Ozen
    • 2
  • Bayram Gocmen
    • 3
  • Ayse Nalbantsoy
    • 2
    Email author
  1. 1.Department of Biology, Basic and Industrial Microbiology Section, Faculty of ScienceEge UniversityBornovaTurkey
  2. 2.Department of Bioengineering, Faculty of EngineeringEge UniversityBornovaTurkey
  3. 3.Department of Biology, Zoology Section, Faculty of ScienceEge UniversityBornovaTurkey

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