, Volume 65, Issue 4, pp 609–619 | Cite as

Role of phytoestrogenic oils in alleviating osteoporosis associated with ovariectomy in rats

  • Hanaa A. Hassan
  • Azza M. EL Wakf
  • Nermin E. EL Gharib
Original Research


The objective of this study was to elucidate the effect of soybean oil (SbO) and sesame oil (SO) supplemented diets on bone biomarkers changes in OVX (ovariectomized) rats. The current data exhibited significant decrease in BMD (bone mineral density), accompanied with marked depletion in the level of Ca, P and Mg in both serum and bone of OVX rats. Also, serum estrogen, total protein, HDL-C (high density lipoprotein cholesterol), bone NO levels were decreased in OVX rats. However, a significant increase in the level of serum TL (total lipids), TC (total cholesterol), TG (triglycerides), LDL-C (low density lipoprotein cholesterol), VLDL-C (very low density lipoprotein cholesterol), urine minerals (Ca, P, Mg), as well as serum, bone and urine ALP (alkaline phosphatase) and ACP (acid phosphatase) activity were recorded in OVX rats. Further changes were also detected by the increased level of urine hydroxyproline, serum parathyroid hormone and osteocalcin, as well as urea and creatinine level in both serum and urine. On the other hand, when OVX rats were fed on SbO (soy bean oil) (15 % w/w) or SO (sesame oil) (10 % w/w) supplemented diets, the data recorded a significant improvement in all the above mentioned parameters. So, it can be concluded that consumption of SbO or SO supplemented diets might be considered as a functional food for retarding risks of osteoporosis associated with estrogen deficiency in OVX states.


Ovariectomyized rat Estrogen deficiency Soybean oil Sesame oil osteoporosis 



Normal control


Soy bean oil


Sesame oil




Bone mineral density


Total lipids


Total cholesterol




High density lipoprotein cholesterol


Low density lipoprotein cholesterol


Very low density lipoprotein cholesterol


Alkaline phosphatase


Acid phosphatase


Nitric oxide








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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Hanaa A. Hassan
    • 1
  • Azza M. EL Wakf
    • 1
  • Nermin E. EL Gharib
    • 1
  1. 1.Physiology Division, Zoology Department, Faculty of ScienceMansoura UniversityMansouraEgypt

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