Cytotechnology

, Volume 59, Issue 2, pp 135–141

Effects of heat treatment and concentration of fish serum on cell growth in adhesion culture of Chinese hamster ovary cells

  • Masashi Fujiwara
  • Ryohei Tsukada
  • Itaru Shioya
  • Mutsumi Takagi
JAACT Special Issue

Abstract

The effects of heat treatment and concentration of fish serum (FS) on cell growth and human granulocyte-macrophage colony-stimulating factor (hGM-CSF) production in an adhesion culture of recombinant Chinese hamster ovary (CHO) cells, DR1000L4N, were investigated. The addition of heat treated FS instead of non-heat-treated FS improved cell growth in terms of cell density, which reached 60% that in 10% fetal calf serum (FCS)-containing medium (FCS medium). A decrease in FS concentration from 10 to 1.25% markedly increased cell density, which was 79% that in 10% FCS medium. The combination of heat treatment at 56 °C and the addition of FS at a low concentration (1.25%) showed an additive effect on cell growth and resulted in the same cell density as that in 10% FCS medium, whereas the hGM-CSF concentration in the culture using FS-containing medium (FS medium) was approximately 50% that in 10% FCS medium. The total lipid concentration in FS was more than three fold that in FCS. The effect of decreasing FS concentration on cell growth may be due to the low lipid concentration in FS medium, because addition of the lipids extracted from FS to 10% FCS and 1.25% FS media markedly decreased cell density. Consequently, the addition of heat-treated FS at low concentrations to medium may be useful for the growth of CHO cells without FCS.

Keywords

Fish serum Chinese hamster ovary cells Lipid Heat treatment Low concentration 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Masashi Fujiwara
    • 1
  • Ryohei Tsukada
    • 1
  • Itaru Shioya
    • 2
  • Mutsumi Takagi
    • 1
  1. 1.Division of Biotechnology and Macromolecular Chemistry, Graduate School of EngineeringHokkaido UniversitySapporoJapan
  2. 2.Central Research Laboratory, Nippon Suisan Kaisha, Ltd.TokyoJapan

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