Quantitative analysis of the responses of murine bone marrow mesenchymal stem cells to EGF, PDGF-BB and fibronectin by factorial design methodology
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Abstract
A 2-level full factorial design was firstly employed to explore the responses of murine bone marrow mesenchymal stem cells stimulated by various combinations of EGF, PDGF-BB, and fibronectin. EGF and PDGF-BB individually, rather than fibronectin, had significant effects on cell proliferation. The synergism between PDGF-BB and fibronectin, as well as the antagonism between EGF and PDGF-BB were also detected. Moreover, cells changed from a flattened and spread to a smaller and elongated shape with addition of factors. The factors also moved cells out of G0/G1 phase, increasing the fractions of cells in S and G2/M phases.
Keywords
Bone marrow mesenchymal stem cells Epidermal growth factor Factorial design Fibronectin Platelet derived growth factor-BBNotes
Acknowledgments
This work was supported by National Nature Sciences Foundation of China (30600332). The authors kindly thank Yantian Chen for ImagePro software analysis and Professor Jianhe Sun for the use of the ELISA reader.
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